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PMID: 226966 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 7 ·1979-07-00 ·Pages 3304-8

Yang HL, Heller K, Gellert M, Zubay G

Abstract

We have used the antibiotics coumermycin A1, novobiocin, and oxolinic acid, which are specific inhibitors of DNA gyrase, to study the coupled transcription and translation of several bacterial and plasmid genes in a DNA-directed cell-free system. The expression of different genes is reduced to different extents by inhibition of DNA gyrase activity. Among the genes tested, the lac operon, an rRNA gene, and the colicin gene of colicin E1 plasmid were found to be most sensitive, while the trp operon and some other genes in colicin E1 plasmid were relatively unaffected by the inhibitors. These results, together with earlier work on the transcription of circular DNA templates, indicate that DNA supercoiling can significantly enhance transcription from certain promoters.

MeSH Terms
Anti-Bacterial Agents/pharmacology Coumarins/pharmacology DNA/metabolism DNA, Circular/metabolism DNA, Superhelical/metabolism Genes/drug effects Novobiocin/pharmacology Oxolinic Acid/pharmacology Plasmids Protein Biosynthesis/drug effects Pyrroles/pharmacology Topoisomerase II Inhibitors Transcription, Genetic/drug effects
Chemicals
Anti-Bacterial Agents Coumarins DNA, Circular DNA, Superhelical Pyrroles Topoisomerase II Inhibitors Novobiocin DNA Oxolinic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yang H L
Heller K
Gellert M
Zubay G
References (29)
29 references, click to expand
  1. Promoter-specific inhibition of transcription by antibiotics which act on DNA gyrase.
    Nature. 1978 Oct 5;275(5679):420-3 PMID: 211433
  2. Contacts between Escherichia coli RNA polymerase and a lac operon promoter.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5314-8 PMID: 364474
  3. Proteins that affect DNA conformation.
    Annu Rev Biochem. 1978;47:449-79 PMID: 209728
  4. Interaction of RNA polymerase with promoters from bacteriophage fd.
    Eur J Biochem. 1977 Mar 15;74(1):107-13 PMID: 300680
  5. An electron microscopic comparison of transcription on linear and superhelical DNA.
    J Mol Biol. 1976 Jul 25;105(1):161-76 PMID: 792457
  6. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4767-71 PMID: 200930
  7. DNA gyrase: an enzyme that introduces superhelical turns into DNA.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3872-6 PMID: 186775
  8. Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4772-6 PMID: 337300
  9. Mapping of a cluster of genes for components of the transcriptional and translational machineries of Escherichia coli.
    J Mol Biol. 1977 Jan 5;109(1):23-47 PMID: 320344
  10. Plasmid detection and sizing in single colony lysates.
    Science. 1977 Jan 28;195(4276):393-4 PMID: 318764
  11. Involvement of DNA gyrase in bacteriophage T7 DNA replication.
    Nature. 1977 Nov 3;270(5632):78-80 PMID: 927524
  12. Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4474-8 PMID: 794878
  13. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455-9 PMID: 4610576
  14. In vitro synthesis of protein in microbial systems.
    Annu Rev Genet. 1973;7:267-87 PMID: 4593305
  15. Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase.
    J Bacteriol. 1973 Nov;116(2):517-26 PMID: 4583237
  16. Angular alteration of the DNA helix by E. coli RNA polymerase.
    Nat New Biol. 1972 Oct 11;239(93):167-70 PMID: 4573694
  17. A possible termination factor for transcription in Escherichia coli.
    Biochem Biophys Res Commun. 1974 Feb 4;56(3):725-31 PMID: 4363749
  18. Detection and isolation of the repressor protein for the tryptophan operon of Escherichia coli.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1100-3 PMID: 4338582
  19. The effect of nalidixic acid on the expression of some genes in Escherichia coli K-12.
    Biochem Biophys Res Commun. 1975 May 5;64(1):204-9 PMID: 167732
  20. Initiation of transcription by Escherichia coli RNA polymerase from supercoiled and non-supercoiled bacteriophage PM2 DNA.
    J Mol Biol. 1975 Feb 5;91(4):477-87 PMID: 1097698
  21. Mechanism of transcription of bacteriophage S13. II. Inhibition of phage-specific transcription by nalidixic acid.
    J Mol Biol. 1973 Mar 25;75(1):99-108 PMID: 4576593
  22. Formation, induction, and curing of bacteriophage P1 lysogens.
    Virology. 1972 Jun;48(3):679-89 PMID: 4555608
  23. Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
    J Mol Biol. 1973 Sep 15;79(2):237-48 PMID: 4760132
  24. Purification and characterization of colicin E2 and colicin E3.
    J Biol Chem. 1967 Nov 25;242(22):5360-8 PMID: 4965137
  25. Template activities of the phi X-174 replicative allomorphic deoxyribonucleic acids.
    Biochemistry. 1971 Nov;10(23):4212-8 PMID: 4942455
  26. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  27. Mode of action of novobiocin in Escherichia coli.
    J Bacteriol. 1967 Jan;93(1):71-9 PMID: 5335903
  28. Synthesis of an R plasmid protein associated with tetracycline resistance is negatively regulated.
    Proc Natl Acad Sci U S A. 1976 May;73(5):1509-12 PMID: 775491
  29. Replication of Escherichia coli DNA in vitro: inhibition by oxolinic acid.
    Eur J Biochem. 1976 Mar 1;62(3):491-7 PMID: 770163
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.7.3304
Published
1979-07-00
Pages
3304-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383813
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.7.3304 PMC Full Text View journal details
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