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PMID: 186775 Published · ppublish English Journal Article

DNA gyrase: an enzyme that introduces superhelical turns into DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 11 ·1976-11-00 ·Pages 3872-6

Gellert M, Mizuuchi K, O'Dea MH, Nash HA

Abstract

Relaxed closed-circular DNA is converted to negatively supercoiled DNA by DNA gyrase. This enzyme has been purified from Escherichia coli cells. The reaction requires ATP and Mg++ and is stimulated by spermidine. The enzyme acts equally well on relaxed closed-circular colicin E1, phage lambda, and simian virus 40 DNA. The final superhelix density of the DNA can be considerably greater than that found in intracellularly supercoiled DNA.

MeSH Terms
Adenosine Triphosphate/metabolism Coliphages DNA, Bacterial/metabolism DNA, Circular/metabolism DNA, Viral/metabolism Enzyme Activation Escherichia coli/enzymology Kinetics Magnesium/pharmacology Nucleic Acid Conformation Plasmids Simian virus 40 Spermidine/pharmacology
Chemicals
DNA, Bacterial DNA, Circular DNA, Viral Adenosine Triphosphate Magnesium Spermidine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gellert M
Mizuuchi K
O'Dea M H
Nash H A
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.11.3872
Published
1976-11-00
Pages
3872-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431247
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.11.3872 PMC Full Text View journal details
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