An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 1 ·1972-01-00 ·Pages 143-6

Champoux JJ, Dulbecco R

Abstract

Nuclei from secondary mouse-embryo cells contain an activity capable of untwisting closed-circular DNAs containing either negative or positive superhelical turns. The activity has no apparent effect on a closed-circular DNA containing no superhelical turns, and is not due to the combined action of an endonuclease and polynucleotide ligase. The enzyme apparently acts by introducing a single-strand nick into the DNA, forming a DNA-enzyme complex that allows the strands to rotate relative to the helix axis before reversing the reaction and sealing the break. The enzyme might possibly serve as a swivel during DNA replication.

MeSH Terms

Animals Carbon Isotopes Cell Nucleus/metabolism Cells, Cultured Centrifugation, Density Gradient Culture Media DNA Replication DNA, Viral/isolation & purification,metabolism Embryo, Mammalian In Vitro Techniques Kidney Mice Nucleic Acid Conformation/drug effects Phenanthridines/pharmacology Polyomavirus/analysis,metabolism Protein Binding Tritium

Chemicals

Carbon Isotopes Culture Media DNA, Viral Phenanthridines Tritium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Champoux J J

Dulbecco R

References (18)

18 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.69.1.143

Published

1972-01-00

Pages

143-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427563

Subset

External Links

PubMed source DOI: 10.1073/pnas.69.1.143 PMC Full Text View journal details

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An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).

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