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PMID: 4070001 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Growth-dependent regulation of rRNA synthesis is mediated by a transcription initiation factor (TIF-IA).

Nucleic acids research ·Vol. 13 ·No. 22 ·1985-11-25 ·Pages 8165-80

Buttgereit D, Pflugfelder G, Grummt I

Abstract

Mouse RNA polymerase I requires at least two chromatographically distinct transcription factors (designated TIF-IA and TIF-IB) to initiate transcription accurately and efficiently in vitro. In this paper we describe the partial purification of TIF-IA by a four-step fractionation procedure. The amount or activity of TIF-IA fluctuates in response to the physiological state of the cells. Extracts from quiescent cells are incapable of specific transcription and do not contain detectable levels of TIF-IA. Transcriptionally inactive extracts can be restored by the addition of TIF-IA preparations that have been highly purified from exponentially growing cells. During the fractionating procedure TIF-IA co-purifies with RNA polymerase I, suggesting that it is functionally associated with the transcribing enzyme. We suggest that only those enzyme molecules that are associated with TIF-IA are capable to interact with TIF-IB and to initiate transcription.

MeSH Terms
Animals Carcinoma, Ehrlich Tumor/analysis Cell Division Cell-Free System Gene Expression Regulation Mice Neoplasm Proteins/isolation & purification,physiology RNA Polymerase I/metabolism RNA, Neoplasm/biosynthesis,genetics RNA, Ribosomal/biosynthesis,genetics Transcription Factors/physiology Transcription, Genetic
Chemicals
Neoplasm Proteins RNA, Neoplasm RNA, Ribosomal Transcription Factors RNA Polymerase I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Buttgereit D
Pflugfelder G
Grummt I
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26 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.22.8165
Published
1985-11-25
Pages
8165-80
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322117
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.22.8165 PMC Full Text View journal details
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