Home LiteratureArticle Details

PMID: 7177852 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor.

Nucleic acids research ·Vol. 10 ·No. 21 ·1982-11-11 ·Pages 6659-70

Mishima Y, Financsek I, Kominami R, Muramatsu M

Abstract

Mouse and human cell extracts (S100) can support an accurate and efficient transcription initiation on homologous ribosomal RNA gene (rDNA) templates. The cell extracts were fractionated with the aid of a phosphocellulose column into four fractions (termed A, B, C and D), including one containing a major part of the RNA polymerase I activity. Various reconstitution experiments indicate that fraction D is an absolute requirement for the correct and efficient transcription initiation by RNA polymerase I on both mouse and human genes. Fraction B effectively suppresses random initiation on these templates. Fraction A appears to further enhance the transcription which takes place with fractions C and D. Although fractions A, B and C are interchangeable between mouse and human extracts, fraction D is not; i.e. initiation of transcription required the presence of a homologous fraction D for both templates. The factor(s) in fraction D, however, is not literally species-specific, since mouse D fraction is capable of supporting accurate transcription initiation on a rat rDNA template in the presence of all the other fractions from human cell extract under the conditions where human D fraction is unable to support it. We conclude from these experiments that a species-dependent factor in fraction D plays an important role in the initiation of rDNA transcription in each animal species.

MeSH Terms

Animals Cloning, Molecular DNA/genetics,isolation & purification DNA, Ribosomal Genes HeLa Cells/metabolism Humans Mammary Neoplasms, Experimental/genetics Mice RNA, Ribosomal/genetics Templates, Genetic Transcription, Genetic

Chemicals

DNA, Ribosomal RNA, Ribosomal DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Mishima Y

Financsek I

Kominami R

Muramatsu M

References (26)

26 references, click to expand

Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium.
Tohoku J Exp Med. 1966 Jan 25;88(1):69-84 PMID: 5920633
Isolation of covalently closed circular DNA of high molecular weight from bacteria.
Anal Biochem. 1976 Dec;76(2):431-41 PMID: 11708
A method for the recovery of DNA from agarose gels.
Nucleic Acids Res. 1978 Jul;5(7):2321-32 PMID: 673856
Adenovirus DNA-directed transcription of 5.5S RNA in vitro.
Proc Natl Acad Sci U S A. 1978 May;75(5):2175-9 PMID: 276858
Molecular analysis of cloned human 18S ribosomal DNA segments.
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5367-71 PMID: 364477
The mechanism of decrease in nucleolar RNA synthesis by protein synthesis inhibition.
J Biochem. 1979 Mar;85(3):807-18 PMID: 429265
Organisation and sequences at the 5' end of a cloned complete ovalbumin gene.
Nature. 1979 Mar 29;278(5703):428-34 PMID: 450048
Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.
Cell. 1979 Oct;18(2):469-84 PMID: 498279
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
Cell. 1980 Jan;19(1):13-25 PMID: 7357599
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
Cell. 1980 Jan;19(1):27-35 PMID: 7357604
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
Cell. 1980 Mar;19(3):717-28 PMID: 6153931
Cloning and determination of a putative promoter region of a mouse ribosomal deoxyribonucleic acid fragment.
Biochemistry. 1980 Aug 5;19(16):3780-6 PMID: 6250576
Promoter sequences of eukaryotic protein-coding genes.
Science. 1980 Sep 19;209(4463):1406-14 PMID: 6251548
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
The structure and evolution of the human beta-globin gene family.
Cell. 1980 Oct;21(3):653-68 PMID: 6985477
Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
J Biol Chem. 1980 Dec 25;255(24):11986-91 PMID: 7440579
Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.
J Biol Chem. 1980 Dec 25;255(24):11992-6 PMID: 7440580
The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.
Nucleic Acids Res. 1980 Dec 20;8(24):6043-58 PMID: 6162156
Specific transcription of mouse ribosomal DNA in a cell-free system that mimics control in vivo.
Proc Natl Acad Sci U S A. 1981 Feb;78(2):727-31 PMID: 6262766
Nucleotide sequence of the initiation site for ribosomal RNA transcription in Drosophila melanogaster: comparison of genes with and without insertions.
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1513-7 PMID: 6262809
Promoter sequence of fibroin gene assigned by in vitro transcription system.
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4838-42 PMID: 6946430
Mapping of a mouse ribosomal DNA promoter by in vitro transcription.
Nucleic Acids Res. 1981 Nov 25;9(22):6093-102 PMID: 6273823
Transcription of mouse rRNA genes by RNA polymerase I: in vitro and in vivo initiation and processing sites.
Cell. 1981 Nov;27(1 Pt 2):165-74 PMID: 7326749
Ribosomal RNA transcription in vitro is species specific.
Nature. 1982 Mar 11;296(5853):173-4 PMID: 7063022
In vitro transcription of a cloned mouse ribosomal RNA gene.
Nucleic Acids Res. 1981 Dec 21;9(24):6773-85 PMID: 6278446
Human ribosomal RNA gene: nucleotide sequence of the transcription initiation region and comparison of three mammalian genes.
Proc Natl Acad Sci U S A. 1982 May;79(10):3092-6 PMID: 6954460

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.21.6659

Published

1982-11-11

Pages

6659-70

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326955

Subset

External Links

PubMed source DOI: 10.1093/nar/10.21.6659 PMC Full Text View journal details

Analysis Services

Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor.

Contact

WeChat Official Account