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PMID: 6262766 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Specific transcription of mouse ribosomal DNA in a cell-free system that mimics control in vivo.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 2 ·1981-02-00 ·Pages 727-31

Grummt I

Abstract

Cloned ribosomal DNA (rDNA) from mouse, which contains the initiation site of 45S pre-rRNA transcription and 5' flanking sequences, has been used as the template in an in vitro transcription system. In the presence of extracts from rapidly growing Ehrlich ascites cells, RNA polymerase I initiates specifically in that region of purified rDNA where the 5' end of 45S rRNA has been mapped. This is shown by electrophoretic analysis of the length of run-off transcripts synthesized from truncated templates, by S1 nuclease mapping, and by hybridization analysis of the in vitro products. The ability of the crude extracts to promote faithful transcription of mouse rDNA correlates with the proliferation rate of the cells. Only extracts prepared from exponentially growing mouse cells contain the factor(s) required for the faithful transcription of mouse ribosomal genes. Extracts from nongrowing or slowly growing mouse cells show very little activity. Thus, the cell-free system somehow reflects the rRNA synthetic activity of the cell and will prove valuable for the identification and purification of the various factors that are involved in the specific read-out of rDNA and may play a central role in the regulation of transcription of the ribosomal genes.

MeSH Terms
Animals Bacteriophage lambda/metabolism Base Sequence Carcinoma, Ehrlich Tumor/metabolism Cell Division Cell-Free System Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant DNA, Ribosomal Mice Plasmids RNA Polymerase I/metabolism RNA, Ribosomal/genetics Transcription, Genetic
Chemicals
DNA, Recombinant DNA, Ribosomal RNA, Ribosomal RNA Polymerase I DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Grummt I
References (31)
31 references, click to expand
  1. The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
    Cell. 1979 Oct;18(2):485-99 PMID: 498280
  2. The structure of the yeast ribosomal RNA genes. 2. The nucleotide sequence of the initiation site for ribosomal RNA transcription.
    Nucleic Acids Res. 1980 Nov 11;8(21):4919-26 PMID: 7003545
  3. Structural analysis of nucleolar precursors of ribosomal ribonucleic acid. Studies on the 5'-terminal and alkali-resistant dinucleotides of nucleolar high molecular weight ribonucleic acid.
    J Biol Chem. 1970 Apr 25;245(8):1954-61 PMID: 4314934
  4. Evidence for the existence of several molecular species in the "45S fraction" of mammalian ribosomal precursor RNA.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1117-20 PMID: 5288361
  5. The rapid turnover of RNA polymerase of rat liver nucleolus, and of its messenger RNA.
    Proc Natl Acad Sci U S A. 1972 Oct;69(10):2833-7 PMID: 4507607
  6. Prolonged transcription in a cell-free system involving nuclei and cytoplasm.
    Proc Natl Acad Sci U S A. 1974 May;71(5):1803-7 PMID: 4525463
  7. Studies on the control of ribosomal RNA synthesis in HeLa cells.
    Eur J Biochem. 1975 Sep 1;57(1):79-83 PMID: 1175642
  8. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  9. Control of ribonucleic acid synthesis in eukaryotes. 3. The effect of cycloheximide and edeine on rna synthesis in yeast.
    Biochemistry. 1976 May 18;15(10):2082-6 PMID: 776214
  10. Amino acid starvation affects the initiation frequency of nucleolar RNA polymerase.
    Cell. 1976 Mar;7(3):439-45 PMID: 947551
  11. The primary ribosomal DNA transcript in eukaryotes.
    Prog Biophys Mol Biol. 1977;31(3):247-69 PMID: 140416
  12. Heterogeneity of 5' -termini of nucleolar 45S, 32S and 28S RNA in mouse hepatoma.
    Nucleic Acids Res. 1977 Jan;4(1):229-40 PMID: 194222
  13. 5'-Terminal capping of RNA by guanylyltransferase from HeLa cell nuclei.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3758-61 PMID: 269428
  14. Ribosomal RNA genes of Saccharomyces cerevisiae. II. Physical map and nucleotide sequence of the 5 S ribosomal RNA gene and adjacent intergenic regions.
    J Biol Chem. 1977 Nov 25;252(22):8126-35 PMID: 334775
  15. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  16. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  17. Sites of transcription initiation in vivo on Xenopus laevis ribosomal DNA.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5402-6 PMID: 271962
  18. Adenovirus DNA-directed transcription of 5.5S RNA in vitro.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2175-9 PMID: 276858
  19. Coincidence of the promoter and capped 5' terminus of RNA from the adenovirus 2 major late transcription unit.
    Cell. 1978 Dec;15(4):1463-75 PMID: 729003
  20. Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
    J Biol Chem. 1979 Jul 10;254(13):6163-73 PMID: 447704
  21. Characterization of a cloned ribosomal fragment from mouse which contains the 18S coding region and adjacent spacer sequences.
    Nucleic Acids Res. 1979 Apr;6(4):1351-69 PMID: 377227
  22. Sequence organization of the spacer DNA in a ribosomal gene unit of Xenopus laevis.
    Cell. 1979 May;17(1):19-31 PMID: 455459
  23. Identification of initiation sites for the in vitro transcription of rRNA operons rrnE and rrnA in E. coli.
    Cell. 1979 May;17(1):211-24 PMID: 378406
  24. Tandem promoters direct E. coli ribosomal RNA synthesis.
    Cell. 1979 May;17(1):225-34 PMID: 110460
  25. The putative promoter of a Xenopus laevis ribosomal gene is reduplicated.
    Nucleic Acids Res. 1979 Aug 24;6(12):3733-43 PMID: 493120
  26. Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.
    Cell. 1979 Oct;18(2):469-84 PMID: 498279
  27. Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes.
    Nature. 1980 Jun 5;285(5764):367-73 PMID: 7383157
  28. Construction and properties of a new cloning vehicle, allowing direct screening for recombinant plasmids.
    Mol Gen Genet. 1980;178(2):475-7 PMID: 6248731
  29. Accurate transcription initiation on a purified mouse beta-globin DNA fragment in a cell-free system.
    Cell. 1980 Jul;20(3):691-9 PMID: 7418005
  30. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855-9 PMID: 6933441
  31. Structural organization of mouse rDNA: comparison of transcribed and non-transcribed regions.
    Mol Gen Genet. 1980 Jan;177(2):223-9 PMID: 6245336
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.2.727
Published
1981-02-00
Pages
727-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319875
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.78.2.727 PMC Full Text View journal details
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