Characterization of a cloned ribosomal fragment from mouse which contains the 18S coding region and adjacent spacer sequences.

Nucleic acids research ·Vol. 6 ·No. 4 ·1979-04-00 ·Pages 1351-69

Grummt I, Soellner C, Scholz I

Abstract

The large EcoRI fragment of mouse ribosomal genes containing parts of the non-transcribed spacer, the external transcribed spacer located at the 5' end of the precursor molecule and about two thirds of the 18S sequence has been cloned in bacteriophage lambda gtWES. A physical map of the DNA was constructed by cleavage with several restriction endonucleases and hybridization of the restriction fragments of the recombinant DNA with labelled 18S and 45S rRNA. The orientation of the inserted fragment as well as the length of the 18S sequence was determined by electron microscopy of R-loop containing molecules. The absence of hybridization of the cloned fragment to other fragments in the genome shows that the non-transcribed spacer does not have a significant length of sequences in common with other sequences in the genome.

MeSH Terms

Animals Base Sequence Carcinoma, Ehrlich Tumor/metabolism Coliphages/metabolism DNA Restriction Enzymes DNA, Recombinant/metabolism Escherichia coli/metabolism Genetic Code Mice Nucleic Acid Hybridization RNA, Ribosomal/biosynthesis Ribosomes/metabolism Transcription, Genetic

Chemicals

DNA, Recombinant RNA, Ribosomal DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Grummt I

Soellner C

Scholz I

References (28)

28 references, click to expand

ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACID.
Biochem J. 1965 Jul;96:266-9 PMID: 14343142
A comparison of two cloned mouse beta-globin genes and their surrounding and intervening sequences.
Cell. 1978 Jun;14(2):237-45 PMID: 566620
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
Synthesis of hybrid bacterial plasmids containing highly repeated satellite DNA.
Cell. 1977 Mar;10(3):509-19 PMID: 403010
The sequence arrangement of Drosophila melanogaster 5s DNA cloned in recombinant plasmids.
Cell. 1977 Jul;11(3):585-98 PMID: 407004
The structural organization of ribosomal DNA in Drosophila melanogaster.
Cell. 1977 Feb;10(2):193-212 PMID: 402222
Cloning of the yeast ribosomal DNA repeat unit in SstI and HindIII lambda vectors using genetic and physical size selections.
J Mol Biol. 1978 Aug 15;123(3):371-86 PMID: 357736
Deletions within E. coli plasmids carrying yeast rDNA.
Gene. 1978 Apr;3(2):135-47 PMID: 350712
Construction and restriction endonuclease mapping of hybrid plasmids containing Saccharomyces cerevisiae ribosomal DNA.
Mol Gen Genet. 1977 Mar 16;151(3):229-44 PMID: 325373
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. I. The AT-rich spacer.
Cell. 1978 Apr;13(4):701-16 PMID: 566163
A very large repeating unit of mouse DNA containing the 18S, 28S and 5.8S rRNA genes.
Cell. 1977 Aug;11(4):795-805 PMID: 560915
Restriction analysis of the nontranscribed spacers of Xenopus laevis ribosomal DNA.
Cell. 1977 Jul;11(3):599-607 PMID: 560255
High capacity gel preparative electrophoresis for purification of fragments of genomic DNA.
Anal Biochem. 1978 Jul 1;87(2):397-410 PMID: 686360
Purification and cloning of a mouse ribosomal gene fragment in coliphage lambda.
Gene. 1977;2(3-4):173-91 PMID: 608592
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
The molecular basis for length heterogeneity in ribosomal DNA from Xenopus laevis.
J Mol Biol. 1976 Aug 25;105(4):461-86 PMID: 787539
Nucleotide sequence of the rightward operator of phage lambda.
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
A short lived polypeptide component of one of two discrete functional pools of hepatic nuclear alpha-amanitin resistant RNA polymerases.
Biochem Biophys Res Commun. 1974 Jun 18;58(4):1030-8 PMID: 4406947
Amino acid starvation affects the initiation frequency of nucleolar RNA polymerase.
Cell. 1976 Mar;7(3):439-45 PMID: 947551
Isolation of high-molecular-weight DNA from mammalian cells.
Eur J Biochem. 1973 Jul 2;36(1):32-8 PMID: 4200179
Sequence studies of the 5 S DNA of Xenopus laevis.
J Mol Biol. 1974 Nov 15;89(4):703-18 PMID: 4449128
Iodination of nucleic acids in vitro.
Biochemistry. 1971 May 25;10(11):1993-2000 PMID: 4935105
Control of ribonucleic acid synthesis in eukaryotes. 3. The effect of cycloheximide and edeine on rna synthesis in yeast.
Biochemistry. 1976 May 18;15(10):2082-6 PMID: 776214
Ribosomal RNA gene dosage as a function of tissue and age for mouse and human.
Biochim Biophys Acta. 1976 Feb 5;418(3):358-75 PMID: 1247550
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
Studies on the control of ribosomal RNA synthesis in HeLa cells.
Eur J Biochem. 1975 Sep 1;57(1):79-83 PMID: 1175642
A membrane-filter technique for the detection of complementary DNA.
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.4.1351

Published

1979-04-00

Pages

1351-69

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327777

Subset

External Links

PubMed source DOI: 10.1093/nar/6.4.1351 PMC Full Text View journal details

Analysis Services

Characterization of a cloned ribosomal fragment from mouse which contains the 18S coding region and adjacent spacer sequences.

Contact

WeChat Official Account