Home LiteratureArticle Details
PMID: 6248731 Published · ppublish English Journal Article

Construction and properties of a new cloning vehicle, allowing direct screening for recombinant plasmids.

Molecular & general genetics : MGG ·Vol. 178 ·No. 2 ·1980-00-00 ·Pages 475-7

Rüther U

Abstract

pUR2, a certified B2(EK2) vector, allows easy isolation of variants containing cloned EcoRI-fragments. Bacteria harboring plasmids without inserts make blue colonies on indicator-plates, whilst those harboring recombinant plasmids make white colonies.

MeSH Terms
Cloning, Molecular Escherichia coli/genetics Genetic Vectors Plasmids Recombination, Genetic
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Rüther U
References (10)
10 references, click to expand
  1. Genetic mapping of the regulator and operator genes of the lac operon.
    J Mol Biol. 1968 Sep 28;36(3):413-7 PMID: 4939632
  2. Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites.
    Nature. 1978 Mar 23;272(5651):375-7 PMID: 416358
  3. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.
    Nucleic Acids Res. 1978 Aug;5(8):2721-8 PMID: 358147
  4. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  5. Characterization by in vitro complementation of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural gene of Escherichia coli.
    J Mol Biol. 1967 Mar 14;24(2):339-43 PMID: 5339877
  6. Molecular basis of beta-galactosidase alpha-complementation.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1254-7 PMID: 1093175
  7. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3642-6 PMID: 333444
  8. Amino acid sequence of beta-galactosidase. IV. Sequence of an alpha-complementing cyanogen bromide peptide, residues 3 to 92.
    J Biol Chem. 1975 Apr 10;250(7):2587-92 PMID: 1091637
  9. Calcium-dependent bacteriophage DNA infection.
    J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
  10. Replica plating and indirect selection of bacterial mutants.
    J Bacteriol. 1952 Mar;63(3):399-406 PMID: 14927572
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00270503
Published
1980-00-00
Pages
475-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00270503
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2