Evidence for the existence of several molecular species in the "45S fraction" of mammalian ribosomal precursor RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 6 ·1971-06-00 ·Pages 1117-20

Tiollais P, Galibert F, Boiron M

Abstract

Analysis of RNA (after 5 or 60 min of labeling with [(3)H]uridine) from L5178Y cells by electrophoresis in 1.7% polyacrylamide gels demonstrates that the ribosomal RNA "45S fraction" is not homogeneous but consists of at least three molecular species, conventionally designated 47S RNA, 46S RNA, and 45S RNA. Alternatives to the classical scheme for the biosynthesis of ribosomal RNA in mammalian cells are discussed.

MeSH Terms

Autoradiography Carbon Isotopes Cells, Cultured/analysis,metabolism Dactinomycin/pharmacology Electrophoresis, Disc HeLa Cells/analysis Molecular Weight RNA, Ribosomal/analysis,biosynthesis Tritium Uridine/metabolism

Chemicals

Carbon Isotopes RNA, Ribosomal Tritium Dactinomycin Uridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Tiollais P

Galibert F

Boiron M

References (15)

15 references, click to expand

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Effects of valine deprivation on the biosynthesis of ribosomal and messenger RNA in a mammalian cell line (L 5178 Y).
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Methylation of 45 s ribosomal RNA precursor in HeLa cells.
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Processing of 45 s nucleolar RNA.
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Selective inhibition of ribosomal RNA synthesis in mammalian cells.
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J Cell Biol. 1969 May;41(2):510-20 PMID: 5783871
Relationship between HeLa cell ribosomal RNA and its precursors studied by high resolution RNA-DNA hybridization.
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Science. 1959 Aug 21;130(3373):432-7 PMID: 13675766
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Composition of nucleoli isolated from mammalian cells.
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Annu Rev Biochem. 1970;39:183-226 PMID: 4920820
Acrylamide gel electrophoresis of HeLa cell nucleolar RNA.
Proc Natl Acad Sci U S A. 1967 Sep;58(3):1088-95 PMID: 5233837
Studies of the culture of leukemic cells in vitro.
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THE CELLULAR SITES OF SYNTHESIS OF RIBOSOMAL AND 4S RNA.
Proc Natl Acad Sci U S A. 1962 Dec;48(12):2179-86 PMID: 16591032
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.6.1117

Published

1971-06-00

Pages

1117-20

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389132

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.6.1117 PMC Full Text View journal details

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Evidence for the existence of several molecular species in the "45S fraction" of mammalian ribosomal precursor RNA.

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