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PMID: 194222 Published · ppublish English Journal Article

Heterogeneity of 5' -termini of nucleolar 45S, 32S and 28S RNA in mouse hepatoma.

Nucleic acids research ·Vol. 4 ·No. 1 ·1977-01-00 ·Pages 229-40

Kominami R, Muramatsu M

Abstract

The 5'-termini of nucleolar 45S, 32S and 28S RNA's were analyzed by means of thin layer chromatography and Dowex-1 colum chromatography. 45S RNA did not bear a triphosphate at the 5'-terminus, but various monophosphates are found. 5'-termini of 32S and 28S RNA's were also heterogeneous. These results indicate that 45S molecules as isolated with the conventional procedure may not contains the primary transcript of the ribosomal gene, but a collection of large precursors with different degrees of processing at the 5'-terminus. The processing of the primary transcript may thus involve some unknown trimming processes at the 5'-terminus before the first major cleavage takes place.

MeSH Terms
Animals Carcinoma, Hepatocellular/analysis Cell Line Cell Nucleolus/analysis Chemical Phenomena Chemistry Liver Neoplasms Mice Neoplasms, Experimental RNA, Neoplasm/isolation & purification RNA, Ribosomal/analysis Ribonucleotides/analysis Ribosomes/analysis Transcription, Genetic
Chemicals
RNA, Neoplasm RNA, Ribosomal Ribonucleotides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kominami R
Muramatsu M
References (28)
28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.1.229
Published
1977-01-00
Pages
229-40
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342422
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.1.229 PMC Full Text View journal details
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