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PMID: 6095193 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In vitro evidence that eukaryotic ribosomal RNA transcription is regulated by modification of RNA polymerase I.

Nucleic acids research ·Vol. 12 ·No. 21 ·1984-11-12 ·Pages 8161-80

Paule MR, Iida CT, Perna PJ, Harris GH, Knoll DA, D'Alessio JM

Abstract

We have utilized a cell-free transcription system from Acanthamoeba castellanii to test the functional activity of RNA polymerase I and transcription initiation factor I (TIF-I) during developmental down regulation of rRNA transcription. The results strongly suggest that rRNA transcription is regulated by modification, probably covalent, of RNA polymerase I: (1) The level of activity of TIF-I in extracts from transcriptionally active and inactive cells is constant. (2) The number of RNA polymerase I molecules in transcriptionally active and inactive cells is also constant. (3) In contrast, though the specific activity of polymerase I on damaged templates remains constant, both crude and purified polymerase I from inactive cells have lost the ability to participate in faithful initiation of rRNA transcription. (4) Polymerase I purified from transcriptionally active cells has the same subunit architecture as enzyme from inactive cells. However, the latter is heat denatured 5 times faster than the active polymerase.

MeSH Terms
Amoeba/genetics,growth & development Animals DNA Restriction Enzymes DNA, Ribosomal/genetics Kinetics RNA Polymerase I/isolation & purification,metabolism RNA, Ribosomal/genetics Thermodynamics Transcription, Genetic
Chemicals
DNA, Ribosomal RNA, Ribosomal RNA Polymerase I DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Paule M R
Iida C T
Perna P J
Harris G H
Knoll D A
D'Alessio J M
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.21.8161
Published
1984-11-12
Pages
8161-80
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320293
Subset
IM
Grants
NIGMS NIH HHS · GM22580 · United States
External Links
PubMed source DOI: 10.1093/nar/12.21.8161 PMC Full Text View journal details
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