Home LiteratureArticle Details
PMID: 4008439 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Organization and nucleotide sequence analysis of an rRNA and tRNA gene cluster from Caulobacter crescentus.

Journal of bacteriology ·Vol. 163 ·No. 1 ·1985-07-00 ·Pages 155-66

Feingold J, Bellofatto V, Shapiro L, Amemiya K

Abstract

rRNA genes of Caulobacter crescentus CB13 were isolated and shown to be present in two gene clusters in the genome. The organization of each rRNA gene cluster was found to be 5'-16S-tRNA spacer-23S-5S-3'. The DNA sequence of 40% of the 16S rRNA gene, the entire 16S/23S intergenic spacer region, and portions of the 23S rRNA gene were determined. Analysis of the nucleotide sequence in the 16S-23S intergenic spacer region revealed the presence of tRNAIle and tRNAAla genes. Large invert repeat sequences were found surrounding the 16S rRNA gene. These inverted repeat sequences are analogous to the RNase III-processing sites in the E. coli rRNA precursor. Small invert repeat sequences were also found flanking the individual tRNA genes. RNA polymerase-binding studies with restriction fragments of the rRNA gene cluster revealed three regions which bound enzyme, and these regions were shown to contain transcription initiation sites. One of these sites was located within the 16S gene near its 3' end, and the other two were found at the 5' end of the 23S gene.

MeSH Terms
Base Sequence Chromosome Mapping DNA, Bacterial/genetics DNA, Ribosomal/genetics Gene Expression Regulation Genes, Bacterial Genetic Linkage Gram-Negative Bacteria/genetics Nucleic Acid Conformation Promoter Regions, Genetic RNA Processing, Post-Transcriptional RNA, Ribosomal/genetics RNA, Transfer/genetics Transcription, Genetic
Chemicals
DNA, Bacterial DNA, Ribosomal RNA, Ribosomal RNA, Transfer
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Feingold J
Bellofatto V
Shapiro L
Amemiya K
References (34)
34 references, click to expand
  1. The phylogeny of prokaryotes.
    Science. 1980 Jul 25;209(4455):457-63 PMID: 6771870
  2. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  3. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  4. Regulation of the synthesis of ribosomes and ribosomal components.
    Annu Rev Biochem. 1984;53:75-117 PMID: 6206783
  5. Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids.
    Microbiol Rev. 1983 Dec;47(4):621-69 PMID: 6363901
  6. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  7. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis.
    Biochemistry. 1967 Jun;6(6):1818-27 PMID: 6035921
  8. Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes.
    Cell. 1981 Jul;25(1):203-13 PMID: 7023695
  9. Transfer RNA genes associated with the 16S and 23S rRNA genes of Euglena chloroplast DNA.
    Biochem Biophys Res Commun. 1980 Jul 16;95(1):47-54 PMID: 6774711
  10. Processing of procaryotic ribonucleic acid.
    Microbiol Rev. 1981 Dec;45(4):502-41 PMID: 6173734
  11. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.
    Biochemistry. 1970 Oct 27;9(22):4428-40 PMID: 4919547
  12. Physical mapping of the ribosomal RNA genes of Mycoplasma capricolum.
    Nucleic Acids Res. 1984 Mar 12;12(5):2421-6 PMID: 6324119
  13. Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.
    J Mol Biol. 1981 May 15;148(2):107-27 PMID: 7028991
  14. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  15. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  16. Purification and characterization of an RNA processing enzyme from Caulobacter crescentus.
    J Biol Chem. 1983 May 10;258(9):5467-76 PMID: 6343387
  17. Isolation, genetic mapping and some characterization of a mutation in Escherichia coli that affects the processing of ribonuleic acid.
    Genetics. 1978 Dec;90(4):659-71 PMID: 369943
  18. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  19. Isolation and mapping of ribosomal RNA genes of Caulobacter crescentus.
    J Mol Biol. 1981 Dec 5;153(2):291-303 PMID: 6279875
  20. tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis.
    Nucleic Acids Res. 1982 Mar 11;10(5):1607-24 PMID: 6280153
  21. Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3593-7 PMID: 358189
  22. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  23. Bacteriophage T7 early promoters: nucleotide sequences of two RNA polymerase binding sites.
    J Mol Biol. 1975 Dec 15;99(3):419-43 PMID: 765476
  24. Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections.
    Science. 1978 Dec 22;202(4374):1279-84 PMID: 725603
  25. The number, physical organization and transcription of ribosomal RNA cistrons in an archaebacterium: Halobacterium halobium.
    Nucleic Acids Res. 1979 Nov 10;7(5):1321-33 PMID: 514815
  26. Membrane phospholipid composition of Caulobacter crescentus.
    J Bacteriol. 1978 Sep;135(3):1130-6 PMID: 690071
  27. Expression of rRNA and tRNA genes in Escherichia coli: evidence for feedback regulation by products of rRNA operons.
    Cell. 1983 Jul;33(3):865-76 PMID: 6191870
  28. BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP.
    Bacteriol Rev. 1964 Sep;28:231-95 PMID: 14220656
  29. Isolation of a Caulobacter gene cluster specifying flagellum production by using nonmotile Tn5 insertion mutants.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6797-801 PMID: 16593248
  30. Ribosomal RNA precursors of Bacillus subtilis.
    Nucleic Acids Res. 1983 Oct 11;11(19):6709-21 PMID: 6314255
  31. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  32. Determinant of cistron specificity in bacterial ribosomes.
    Nature. 1975 Mar 6;254(5495):34-8 PMID: 803646
  33. Differential template recognition by the Caulobacter crescentus and the escherichia coli RNA polymerases.
    J Biol Chem. 1983 Jul 25;258(14):8984-92 PMID: 6306009
  34. Nucleotide sequence of the 16S - 23S spacer region in an rRNA gene cluster from tobacco chloroplast DNA.
    Nucleic Acids Res. 1982 Apr 24;10(8):2665-76 PMID: 6281739
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.163.1.155-166.1985
Published
1985-07-00
Pages
155-66
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC219093
Subset
IM
Grants
NIGMS NIH HHS · GM 11301 · United States
NIGMS NIH HHS · GM 32506 · United States
NCI NIH HHS · P30-CA1-13330 · United States
Databases
GENBANK
L00194, L00195, L00196, M11453
External Links
PubMed source DOI: 10.1128/jb.163.1.155-166.1985 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2