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PMID: 6324119 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Physical mapping of the ribosomal RNA genes of Mycoplasma capricolum.

Nucleic acids research ·Vol. 12 ·No. 5 ·1984-03-12 ·Pages 2421-6

Glaser G, Amikam D, Razin S

Abstract

Physical mapping of the rRNA genes of Mycoplasma capricolum was done by digestion of the mycoplasmal DNA with EcoRI, PstI and BglII and hybridization with nick-translated probes consisting of defined portions of the rrnB ribosomal RNA operon of Escherichia coli. The results indicate that the rRNA genes in the chromosome of M. capricolum are arranged in two clusters, each organized in the order 5'-16S-23S-5S-3', resembling the order of the genes in the rrnB operon, with no large spacer regions separating the genes in each cluster.

MeSH Terms
Base Sequence DNA/genetics DNA Restriction Enzymes DNA, Ribosomal Escherichia coli/genetics Genes Genes, Bacterial Mycoplasma/genetics Nucleic Acid Hybridization Operon Plasmids Protein Biosynthesis RNA, Ribosomal/genetics
Chemicals
DNA, Ribosomal RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Glaser G
Amikam D
Razin S
References (11)
11 references, click to expand
  1. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  2. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  3. ColE1 cloning of a ribosomal RNA promoter region from lambdarifd18 by selection for lambda integration and excision functions.
    Gene. 1977;2(3-4):159-72 PMID: 344139
  4. Intragenic DNA spacers interrupt the ovalbumin gene.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1299-303 PMID: 274719
  5. Preparative and analytical purification of DNA from agarose.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9 PMID: 284385
  6. Methylation of foreign DNA sequences in eukaryotic cells.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6463-7 PMID: 6935661
  7. Stable RNA synthesis and its control in Mycoplasma capricolum.
    Nucleic Acids Res. 1981 Aug 11;9(15):3641-6 PMID: 6169010
  8. Construction and fine mapping of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli.
    Plasmid. 1981 Jul;6(1):112-8 PMID: 7025054
  9. The number of ribosomal RNA genes in Mycoplasma capricolum.
    Mol Gen Genet. 1981;182(3):502-4 PMID: 6272066
  10. Ribosomal RNA genes in Mycoplasma.
    Nucleic Acids Res. 1982 Jul 24;10(14):4215-22 PMID: 6289267
  11. Organization of rRNA structural genes in the archaebacterium Thermoplasma acidophilum.
    Nucleic Acids Res. 1982 Nov 25;10(22):7231-45 PMID: 7155894
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.5.2421
Published
1984-03-12
Pages
2421-6
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318672
Subset
IM
Grants
NIGMS NIH HHS · GM25286 · United States
External Links
PubMed source DOI: 10.1093/nar/12.5.2421 PMC Full Text View journal details
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