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PMID: 6281739 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the 16S - 23S spacer region in an rRNA gene cluster from tobacco chloroplast DNA.

Nucleic acids research ·Vol. 10 ·No. 8 ·1982-04-24 ·Pages 2665-76

Takaiwa F, Sugiura M

Abstract

The nucleotide sequence of a spacer region between 16S and 23S rRNA genes from tobacco chloroplasts has been determined. The spacer region is 2080 bp long and encodes tRNAIle and tRNAAla genes which contain intervening sequences of 707 bp and 710 bp, respectively. Strong homology between the two intervening sequences is observed. These spacer tRNAs are synthesized as part of an 8.2 kb precursor molecule containing 16S and 23S rRNA sequences.

MeSH Terms
Base Composition Base Sequence Chloroplasts/metabolism DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Nucleic Acid Hybridization Plants/genetics Plants, Toxic Plasmids RNA, Ribosomal/genetics RNA, Transfer/genetics Tobacco/genetics
Chemicals
DNA, Recombinant RNA, Ribosomal RNA, Transfer DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Takaiwa F
Sugiura M
References (24)
24 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. Sequencing of 16S--23S spacer in a ribosomal RNA operon of Euglena gracilis chloroplast DNA reveals two tRNA genes.
    Nature. 1980 Aug 28;286(5776):908-10 PMID: 6774264
  3. The structure and evolution of the human beta-globin gene family.
    Cell. 1980 Oct;21(3):653-68 PMID: 6985477
  4. Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli.
    J Mol Biol. 1978 Dec 25;126(4):847-57 PMID: 370408
  5. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  6. Analysis of a drosophila tRNA gene cluster: two tRNALeu genes contain intervening sequences.
    Cell. 1981 Jan;23(1):251-9 PMID: 6783316
  7. Overlapping divergent genes in the maize chloroplast chromosome and in vitro transcription of the gene for tRNA.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3423-7 PMID: 16593030
  8. Cloning and characterization of 4.5S and 5S RNA genes in tobacco chloroplasts.
    Gene. 1980 Jul;10(2):95-103 PMID: 6248424
  9. Splicing of yeast tRNA precursors: structure of the reaction intermediates.
    Cell. 1979 Sep;18(1):37-45 PMID: 389432
  10. Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes.
    Cell. 1981 Jul;25(1):203-13 PMID: 7023695
  11. The nucleotide sequences of two tRNAAsn genes from tobacco chloroplasts.
    Nucleic Acids Res. 1981 Nov 11;9(21):5601-7 PMID: 6273800
  12. Plastid DNA-the plastome.
    Results Probl Cell Differ. 1980;10:45-96 PMID: 6999574
  13. Localization of 4S RNA genes on the chloroplast genome of Chlamydomonas reinhardii.
    Mol Gen Genet. 1978 Nov 9;166(3):269-75 PMID: 739975
  14. Order and intracellular location of the events involved in the maturation of a spliced tRNA.
    Nature. 1980 Mar 13;284(5752):143-8 PMID: 6987526
  15. Isolation of yeast tRNALeu genes. DNA sequence of a cloned tRNALeu3 gene.
    J Biol Chem. 1979 Dec 25;254(24):12306-9 PMID: 387786
  16. Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):2710-4 PMID: 331313
  17. The rRNA operon from Zea mays chloroplasts: nucleotide sequence of 23S rDNA and its homology with E.coli 23S rDNA.
    Nucleic Acids Res. 1981 Jun 25;9(12):2853-69 PMID: 7024906
  18. Presence of a transfer RNA gene in the spacer sequence between the 16 S and 23 S rRNA genes of spinach chloroplast DNA.
    FEBS Lett. 1979 Jul 1;103(1):52-6 PMID: 467653
  19. The synthesis of chloroplast high-molecular-weight ribosomal ribonucleic acid in spinach.
    Eur J Biochem. 1979 May 15;96(2):301-9 PMID: 456375
  20. Sequence of the 16 S-23 s spacer region in two ribosomal RNA operons of Escherichia coli.
    J Biol Chem. 1979 May 10;254(9):3264-71 PMID: 372188
  21. Sequence of a putative promoter region for the rRNA genes of tobacco chloroplast DNA.
    Nucleic Acids Res. 1981 Oct 24;9(20):5399-406 PMID: 7029469
  22. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  23. Euglena gracilis chloroplast ribosomal RNA transcription units. II. Nucleotide sequence homology between the 16 S--23 S ribosomal RNA spacer and the 16 S ribosomal RNA leader regions.
    J Biol Chem. 1980 Nov 25;255(22):10997-1003 PMID: 6253497
  24. A gene coding for tRNA is located near 5' terminus of 16S rRNA gene in Zea mays chloroplast genome.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4748-52 PMID: 16593071
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1982-04-24
Pages
2665-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320641
Subset
IM
Databases
GENBANK
J01452
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