Home LiteratureArticle Details
PMID: 6314255 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Ribosomal RNA precursors of Bacillus subtilis.

Nucleic acids research ·Vol. 11 ·No. 19 ·1983-10-11 ·Pages 6709-21

Loughney K, Lund E, Dahlberg JE

Abstract

The DNA sequence of the region corresponding to the 5'-end of a 16S rRNA gene of B. subtilis 168 was determined. Comparison of this sequence with the sequences flanking other 16S and 23S rRNA coding regions (1-4) indicated that large RNA stem structures, surrounding the mature 16S and 23S rRNAs, could form in a precursor rRNA. The 5'-ends of the precursors of 16S and 23S rRNAs (p16S and p23S) were mapped to the middles of these potential RNA stem structures. We propose that the initial cleavages of the primary rRNA transcript occur near the "opposed G's" which interrupt the basepairing of each of these stem structures. This model is supported by the finding that the 5'-end of the 5S rRNA precursor, p5A (5), maps to the region of the "opposed G's" in the 23S rRNA stem structure.

MeSH Terms
Bacillus subtilis/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/genetics Endonucleases Genes, Bacterial Molecular Weight Nucleic Acid Conformation Operon Plasmids RNA, Ribosomal/genetics Single-Strand Specific DNA and RNA Endonucleases
Chemicals
DNA, Bacterial RNA, Ribosomal Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Loughney K
Lund E
Dahlberg J E
References (30)
30 references, click to expand
  1. Genetic and base sequence homologies in bacillus.
    Genetics. 1969 Jul;62(3):697-710 PMID: 4989093
  2. Differential stringent control of the tandem E. coli ribosomal RNA promoters from the rrnA operon expressed in vivo in multicopy plasmids.
    Cell. 1983 Apr;32(4):1337-46 PMID: 6188537
  3. Location of genetic loci of ribosomal RNA on Bacillus subtilis chromosome.
    Proc Natl Acad Sci U S A. 1965 Aug;54(2):483-91 PMID: 4955654
  4. Separation of bacterial ribosomal ribonucleic acid from its macromolecular precursors by polyacrylamide gel electrophoresis.
    J Bacteriol. 1968 Mar;95(3):986-90 PMID: 4966836
  5. Analysis of the 5'-terminal nucleotide sequences of ribonucleic acids. II. Comparison of the 5'-terminal nucleotide sequences of ribosomal RNA's from different organisms.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1595-602 PMID: 4296143
  6. Chromosomal location of DNA base sequences complementary to transfer RNA and to 5 s, 16 s and 23 s ribosomal RNA in Bacillus subtilis.
    J Mol Biol. 1968 Apr 14;33(1):123-40 PMID: 4967203
  7. Stability of ribonucleic acid double-stranded helices.
    J Mol Biol. 1974 Jul 15;86(4):843-53 PMID: 4427357
  8. Nucleotide sequence of 5 S ribosomal RNA precursor from Bacillus subtilis.
    J Biol Chem. 1976 Jun 10;251(11):3480-8 PMID: 179998
  9. Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4145-9 PMID: 825858
  10. Ribosomal RNA genes in Bacillus subtilis. Evidence for a cotranscription mechanism.
    Biochim Biophys Acta. 1977 Feb 16;474(4):562-77 PMID: 402154
  11. Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule.
    J Bacteriol. 1977 Jul;131(1):374-7 PMID: 406259
  12. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  13. Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3593-7 PMID: 358189
  14. Organization of transfer and ribosomal ribonucleic acid genes in Bacillus subtilis.
    J Bacteriol. 1979 Nov;140(2):742-4 PMID: 115849
  15. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  16. Sequence analysis and in vitro maturation of five precursor 5S RNAs from Bacillus Q.
    Nucleic Acids Res. 1980 May 24;8(10):2193-211 PMID: 6159574
  17. Nucleotide sequence analysis of precursor 5S RNA from Bacillus licheniformis.
    Nucleic Acids Res. 1980 Oct 10;8(19):4535-41 PMID: 6159590
  18. Genetic mapping of a linked cluster of ribosomal ribonucleic acid genes in Bacillus subtilis.
    J Bacteriol. 1981 Nov;148(2):624-8 PMID: 6795182
  19. Cloning and analysis of strong promoters is made possible by the downstream placement of a RNA termination signal.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4936-40 PMID: 6946440
  20. Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. I. Isolation and characterization of plasmids containing the origin region.
    Mol Gen Genet. 1981;183(2):220-6 PMID: 6276678
  21. tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis.
    Nucleic Acids Res. 1982 Mar 11;10(5):1607-24 PMID: 6280153
  22. The number of 5 S rRNA genes in Bacillus subtilis.
    FEBS Lett. 1982 May 17;141(2):161-3 PMID: 6284552
  23. Ribosomal RNA genes in the replication origin region of Bacillus subtilis chromosome.
    Nature. 1982 Sep 16;299(5880):268-71 PMID: 6810183
  24. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis.
    Mol Gen Genet. 1982;186(3):339-46 PMID: 6181373
  25. Identification of a suppressor sequence for DNA replication in the replication origin region of the Bacillus subtilis chromosome.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4285-9 PMID: 6812045
  26. A gene between polA and glnA retards growth of Escherichia coli when present in multiple copies: physiological effects of the gene for spot 42 RNA.
    J Bacteriol. 1982 Dec;152(3):1196-210 PMID: 6183252
  27. Detailed physical mapping of the ribosomal RNA genes of Bacillus subtilis.
    Gene. 1982 Sep;19(2):153-62 PMID: 6293924
  28. Structure and organization of a cluster of sic tRNA genes in the space between tandem ribosomal RNA gene sets in Bacillus subtilis.
    J Biol Chem. 1983 Jan 10;258(1):291-8 PMID: 6294101
  29. Replication origin region of Bacillus subtilis chromosome contains two rRNA operons.
    J Bacteriol. 1983 Apr;154(1):50-7 PMID: 6187731
  30. Electron microscope mapping of the distribution of ribosomal genes of the Bacillus subtilis chromosome.
    J Mol Biol. 1973 Apr 5;75(2):265-79 PMID: 4199565
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.19.6709
Published
1983-10-11
Pages
6709-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326409
Subset
IM
Databases
GENBANK
V01489
External Links
PubMed source DOI: 10.1093/nar/11.19.6709 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2