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PMID: 6293924 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Detailed physical mapping of the ribosomal RNA genes of Bacillus subtilis.

Gene ·Vol. 19 ·No. 2 ·1982-09-00 ·Pages 153-62

Stewart GC, Wilson FE, Bott KF

Abstract

Characterization of patterns of ribosomal RNA (rRNA) homology with restriction digests of Bacillus subtilis 168 chromosomal DNA and with cloned DNA sequences has resulted in the construction of a physical map of the rRNA gene sets. There are two types of gene sets which differ in the size of "spacer" DNA sequences separating the 16S and 23S rRNA determinants. It was estimated that there are ten rRNA gene sets on the B. subtilis chromosome.

MeSH Terms
Bacillus subtilis/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes Genes, Bacterial Molecular Weight Nucleic Acid Hybridization RNA, Ribosomal/genetics
Chemicals
RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stewart G C
Wilson F E
Bott K F
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
Published
1982-09-00
Pages
153-62
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
Grants
NIGMS NIH HHS · GM07900 · United States
NIGMS NIH HHS · GM26399 · United States
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