Two highly polymorphic minisatellites from the pseudoautosomal region of the human sex chromosomes.

The EMBO journal ·Vol. 6 ·No. 4 ·1987-04-00 ·Pages 963-9

Simmler MC, Johnsson C, Petit C, Rouyer F, Vergnaud G, Weissenbach J

Abstract

Two pseudoautosomal loci DXYS15 and DXYS17 from the pairing region of the human sex chromosomes display a high variability with at least eight alleles each. The structural elements responsible for the polymorphisms have been isolated and sequenced. In both cases the variations result from DNA rearrangements occurring in tandemly repeated sequences (minisatellites) of 21-29 nucleotides for DXYS15 and 28-33 nucleotides for DXYS17. At reduced stringency, the DXYS15 minisatellite detects other hypervariable sequences located in other parts of the genome and hence represents a new family of minisatellites. In contrast to most other known hypervariable families, the DXYS15 hypervariable sequence displays a very high AT content.

MeSH Terms

Alleles Base Sequence DNA, Satellite/genetics Female Genetic Variation Humans Male Nucleic Acid Hybridization Polymorphism, Genetic Sex Chromosomes

Chemicals

DNA, Satellite

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Simmler M C

Johnsson C

Petit C

Rouyer F

Vergnaud G

Weissenbach J

References (32)

32 references, click to expand

Human-mouse cell hybrid with human multiple Y chromosomes.
Nature. 1976 Jul 1;262(5563):63-5 PMID: 945468
A hypervariable region 3' to the human apolipoprotein B gene.
Nucleic Acids Res. 1986 Nov 25;14(22):9215-6 PMID: 3786150
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
A highly polymorphic locus in human DNA.
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6754-8 PMID: 6935681
Structure of chi hotspots of generalized recombination.
Cell. 1981 May;24(2):429-36 PMID: 6453653
Highly variable regions of DNA flank the human alpha globin genes.
Nucleic Acids Res. 1981 Sep 11;9(17):4213-24 PMID: 6272199
The highly polymorphic region near the human insulin gene is composed of simple tandemly repeating sequences.
Nature. 1982 Jan 7;295(5844):31-5 PMID: 7035959
Variation in the sequence and modification state of the human insulin gene flanking regions.
Nucleic Acids Res. 1982 Apr 10;10(7):2225-40 PMID: 6283472
Genetic homology and crossing over in the X and Y chromosomes of Mammals.
Hum Genet. 1982;61(2):85-90 PMID: 7129448
The structure of the human zeta-globin gene and a closely linked, nearly identical pseudogene.
Cell. 1982 Dec;31(3 Pt 2):553-63 PMID: 6297773
Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.
Nature. 1983 Mar 3;302(5903):33-7 PMID: 6298635
Single-copy DNA sequences specific for the human Y chromosome.
Nature. 1983 Jun 30;303(5920):831-2 PMID: 6866083
Molecular basis of length polymorphism in the human zeta-globin gene complex.
Proc Natl Acad Sci U S A. 1983 Aug;80(16):5022-6 PMID: 6308667
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
A simple and very efficient method for generating cDNA libraries.
Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
Construction of linkage maps with DNA markers for human chromosomes.
Nature. 1985 Jan 10-18;313(5998):101-5 PMID: 2981412
Hypervariable 'minisatellite' regions in human DNA.
Nature. 1985 Mar 7-13;314(6006):67-73 PMID: 3856104
Propagation of some human DNA sequences in bacteriophage lambda vectors requires mutant Escherichia coli hosts.
Proc Natl Acad Sci U S A. 1985 May;82(9):2880-4 PMID: 3157994
Individual-specific 'fingerprints' of human DNA.
Nature. 1985 Jul 4-10;316(6023):76-9 PMID: 2989708
A highly polymorphic region 3' to the human type II collagen gene.
Nucleic Acids Res. 1985 Jul 11;13(13):4613-22 PMID: 4022769
Hypervariable telomeric sequences from the human sex chromosomes are pseudoautosomal.
Nature. 1985 Oct 24-30;317(6039):687-92 PMID: 2997619
Pseudoautosomal DNA sequences in the pairing region of the human sex chromosomes.
Nature. 1985 Oct 24-30;317(6039):692-7 PMID: 2997620
Report of the Committee on the Genetic Constitution of the X and Y Chromosomes.
Cytogenet Cell Genet. 1985;40(1-4):296-352 PMID: 3864598
A gradient of sex linkage in the pseudoautosomal region of the human sex chromosomes.
Nature. 1986 Jan 23-29;319(6051):291-5 PMID: 3941746
Gene organization and recombinational hotspots in the murine major histocompatibility complex.
Cell. 1986 Mar 28;44(6):895-904 PMID: 3754180
Cloning a selected fragment from a human DNA 'fingerprint': isolation of an extremely polymorphic minisatellite.
Nucleic Acids Res. 1986 Jun 11;14(11):4605-16 PMID: 2423969
Structure and evolution of the human involucrin gene.
Cell. 1986 Aug 15;46(4):583-9 PMID: 2873896
Molecular analysis of the hotspot of recombination in the murine major histocompatibility complex.
Science. 1986 Oct 10;234(4773):173-9 PMID: 3018929
Molecular characterisation of a hypervariable region downstream of the human alpha-globin gene cluster.
EMBO J. 1986 Aug;5(8):1857-63 PMID: 3019666
A pseudoautosomal gene in man.
Science. 1986 Nov 7;234(4777):740-3 PMID: 2877492
Molecular characterization of a meiotic recombinational hotspot enhancing homologous equal crossing-over.
EMBO J. 1986 Sep;5(9):2123-9 PMID: 3023057
Synthesis of hybrid bacterial plasmids containing highly repeated satellite DNA.
Cell. 1977 Mar;10(3):509-19 PMID: 403010

Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1987-04-00

Pages

963-9

Language

English

Region

England

NLM ID

8208664

PMCID

PMC553490

Subset

External Links

PubMed source PMC Full Text View journal details

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Two highly polymorphic minisatellites from the pseudoautosomal region of the human sex chromosomes.

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