Abstract
The growth of clones of human genomic DNA fragments in a bacteriophage lambda vector has been examined in a number of different Escherichia coli hosts. A large proportion (8.9%) of the phages carrying different fragments of the human genome fail to grow on standard rec+ hosts but will grow on hosts carrying mutations in the recB, recC, and sbcB genes. Heteroduplex analysis in the electron microscope of DNA from four of these phages revealed substantial secondary structure, including snap-back regions 200-500 base pairs in length. Such structures were not found in phages from the same DNA library that grow in rec+ hosts. These results are interpreted in the light of prior observations [Leach, D.R.F. & Stahl, F. (1983) Nature (London) 305, 448-451] showing that inverted repetitions cloned in phage lambda can be propagated in recB recC sbcB hosts but not in rec+ hosts.
MeSH Terms
Bacteriophage lambda/genetics
Base Sequence
Cloning, Molecular
DNA, Recombinant
Escherichia coli/genetics
Genetic Vectors
Humans
Mutation
Nucleic Acid Heteroduplexes/genetics
Polymorphism, Genetic
Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Recombinant
Nucleic Acid Heteroduplexes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wyman A R
Wolfe L B
Botstein D
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13 references, click to expand
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