Home LiteratureArticle Details
PMID: 3019666 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular characterisation of a hypervariable region downstream of the human alpha-globin gene cluster.

The EMBO journal ·Vol. 5 ·No. 8 ·1986-08-00 ·Pages 1857-63

Jarman AP, Nicholls RD, Weatherall DJ, Clegg JB, Higgs DR

Abstract

We have characterised an unusual, highly polymorphic region of DNA located 8-kb downstream of the human alpha-globin gene complex. This hypervariable region (alpha-globin 3' HVR) is composed of an array of 17-bp tandem repeats, the number of which differs considerably (70-450) from one allele to another. The sequence of the 17-bp repeats is highly conserved within and between alleles. Furthermore, this sequence identifies a core oligonucleotide [5'-GNGGGG(N)ACAG-3'] that is common to three previously characterised hypervariable regions. At reduced stringency, a probe to the 3' HVR detects a new family of multiallelic loci that will be of value in the study of human genetics.

MeSH Terms
Alleles Base Sequence Biological Evolution Cloning, Molecular DNA Restriction Enzymes Genes Genetic Variation Globins/genetics Humans Nucleic Acid Hybridization Repetitive Sequences, Nucleic Acid
Chemicals
Globins DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jarman A P
Nicholls R D
Weatherall D J
Clegg J B
Higgs D R
References (34)
34 references, click to expand
  1. Base sequence and evolution of guinea-pig alpha-satellite DNA.
    Nature. 1970 Aug 22;227(5260):794-8 PMID: 4317352
  2. Mapping genetic elements of Epstein-Barr virus that facilitate extrachromosomal persistence of Epstein-Barr virus-derived plasmids in human cells.
    Mol Cell Biol. 1985 Oct;5(10):2533-42 PMID: 3016506
  3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  4. Evolution of repeated DNA sequences by unequal crossover.
    Science. 1976 Feb 13;191(4227):528-35 PMID: 1251186
  5. A simple method for DNA restriction site mapping.
    Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
  6. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  7. Construction of a genetic linkage map in man using restriction fragment length polymorphisms.
    Am J Hum Genet. 1980 May;32(3):314-31 PMID: 6247908
  8. The chromosomal arrangement of human alpha-like globin genes: sequence homology and alpha-globin gene deletions.
    Cell. 1980 May;20(1):119-30 PMID: 6446404
  9. A highly polymorphic locus in human DNA.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6754-8 PMID: 6935681
  10. Unusual structure of the FB family of transposable elements in Drosophila.
    Cell. 1981 Jun;24(3):753-63 PMID: 6265101
  11. Highly variable regions of DNA flank the human alpha globin genes.
    Nucleic Acids Res. 1981 Sep 11;9(17):4213-24 PMID: 6272199
  12. The highly polymorphic region near the human insulin gene is composed of simple tandemly repeating sequences.
    Nature. 1982 Jan 7;295(5844):31-5 PMID: 7035959
  13. Variation in the sequence and modification state of the human insulin gene flanking regions.
    Nucleic Acids Res. 1982 Apr 10;10(7):2225-40 PMID: 6283472
  14. The structure of the human zeta-globin gene and a closely linked, nearly identical pseudogene.
    Cell. 1982 Dec;31(3 Pt 2):553-63 PMID: 6297773
  15. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.
    Nature. 1983 Mar 3;302(5903):33-7 PMID: 6298635
  16. Somatic generation of antibody diversity.
    Nature. 1983 Apr 14;302(5909):575-81 PMID: 6300689
  17. Molecular basis of length polymorphism in the human zeta-globin gene complex.
    Proc Natl Acad Sci U S A. 1983 Aug;80(16):5022-6 PMID: 6308667
  18. BK viral enhancer element and a human cellular homolog.
    Science. 1983 Nov 18;222(4625):749-55 PMID: 6314501
  19. An unusual transposon with long terminal inverted repeats in the sea urchin Strongylocentrotus purpuratus.
    Nature. 1983 Nov 24-30;306(5941):342-7 PMID: 6316151
  20. Regulation of plasmid replication.
    Microbiol Rev. 1984 Mar;48(1):1-23 PMID: 6201704
  21. Enhancers and ribosomal gene spacers.
    Cell. 1984 Sep;38(2):349-51 PMID: 6467370
  22. The molecular basis of alpha-thalassaemia in Thailand.
    EMBO J. 1984 Aug;3(8):1813-8 PMID: 6548185
  23. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  24. Hypervariable 'minisatellite' regions in human DNA.
    Nature. 1985 Mar 7-13;314(6006):67-73 PMID: 3856104
  25. Fine mapping and sequencing of a variable segment in the inverted repeat region of varicella-zoster virus DNA.
    J Virol. 1985 May;54(2):639-42 PMID: 2985828
  26. Propagation of some human DNA sequences in bacteriophage lambda vectors requires mutant Escherichia coli hosts.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2880-4 PMID: 3157994
  27. Individual-specific 'fingerprints' of human DNA.
    Nature. 1985 Jul 4-10;316(6023):76-9 PMID: 2989708
  28. Allelic variation and linkage properties of a highly polymorphic restriction fragment in humans.
    Mol Biol Med. 1984 Jun;2(3):223-38 PMID: 6100388
  29. A highly polymorphic DNA marker linked to adult polycystic kidney disease on chromosome 16.
    Nature. 1985 Oct 10-16;317(6037):542-4 PMID: 2995836
  30. Positive identification of an immigration test-case using human DNA fingerprints.
    Nature. 1985 Oct 31-Nov 6;317(6040):818-9 PMID: 4058586
  31. Forensic application of DNA 'fingerprints'.
    Nature. 1985 Dec 12-18;318(6046):577-9 PMID: 3840867
  32. Direct cloning of specific genomic DNA sequences in plasmid libraries following fragment enrichment.
    Nucleic Acids Res. 1985 Nov 11;13(21):7569-78 PMID: 2999697
  33. Two genetic markers closely linked to adult polycystic kidney disease on chromosome 16.
    Br Med J (Clin Res Ed). 1986 Mar 29;292(6524):851-3 PMID: 3008903
  34. Long range periodicities in mouse satellite DNA.
    J Mol Biol. 1975 May 5;94(1):51-69 PMID: 1142436
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-08-00
Pages
1857-63
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167051
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2