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PMID: 3520241 Published · ppublish English Journal Article

The xylABC promoter from the Pseudomonas putida TOL plasmid is activated by nitrogen regulatory genes in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 203 ·No. 1 ·1986-04-00 ·Pages 129-36

Dixon R

Abstract

The xylABC promoter (OP1), located on the TOL plasmid of Pseudomonas putida contains sequences homologous to the conserved regions found in nitrogen fixation (nif) promoters and in other promoters subject to nitrogen control. XylA-lac fusions were constructed in order to monitor expression from the OP1 promoter in Escherichia coli. Transcription was activated in the presence of the heterologous regulatory genes ntrC or nifA from Klebsiella pneumoniae as well as by the homologous P. putida regulatory gene xylR. In all cases activation was also dependent on the ntrA gene, whose product has been implicated as a specific sigma factor for ntr activatable operons. The 5' ends of xylA mRNA, detected by S1 nuclease mapping of in vivo transcripts, were identical in strains containing xylR, ntrC or nifA as transcriptional activators. However, activation of the K. pneumoniae nifL or nifH promoters by xylR was not detected.

MeSH Terms
Base Sequence Escherichia coli/genetics Genes, Bacterial Genes, Regulator Genotype Klebsiella pneumoniae/genetics Nitrogen/metabolism Phenotype Plasmids Promoter Regions, Genetic Protein Biosynthesis Pseudomonas/genetics Sequence Homology, Nucleic Acid Species Specificity Transcription, Genetic
Chemicals
Nitrogen
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Dixon R
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51 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330393
Published
1986-04-00
Pages
129-36
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330393
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