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PMID: 2862031 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Nitrogen regulation in Salmonella typhimurium. Identification of an ntrC protein-binding site and definition of a consensus binding sequence.

The EMBO journal ·Vol. 4 ·No. 2 ·1985-02-00 ·Pages 539-47

Ferro-Luzzi Ames G, Nikaido K

Abstract

We have investigated the DNA-binding ability of a nitrogen regulatory protein, the product of the ntrC gene, to several nitrogen-regulated promoters in Salmonella typhimurium. The ntrC protein is able to bind to the regulatory region (dhuA) of an operon coding for genes involved in the active transport of histidine, but not to another transport-related regulatory region, argTr. It bound to two different sites within the regulatory region of glnA (glutamine synthetase) and to one site in the regulatory region for the ntrBC operon. A consensus sequence has been derived from these four binding sites. The binding sequence displays dyad symmetry, as expected for the dimeric ntrC protein. The relationship of the binding sites to regulation of transcription initiation and termination, and to published homologies within the sequences of regulatory sites for nif genes is discussed.

MeSH Terms
Amino Acid Transport Systems Arginine/genetics Bacterial Proteins/genetics Binding Sites DNA, Bacterial/genetics DNA-Binding Proteins/genetics Genes, Regulator Glutamate-Ammonia Ligase/genetics Histidine/genetics Membrane Transport Proteins/genetics Nitrogen/metabolism Operon Salmonella typhimurium/genetics Transcription Factors/genetics Transcription, Genetic
Chemicals
Amino Acid Transport Systems Bacterial Proteins DNA, Bacterial DNA-Binding Proteins Membrane Transport Proteins Transcription Factors Histidine Arginine Glutamate-Ammonia Ligase Nitrogen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ferro-Luzzi Ames G
Nikaido K
References (28)
28 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-02-00
Pages
539-47
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554219
Subset
IM
Grants
NIADDK NIH HHS · AM12121 · United States
External Links
PubMed source PMC Full Text View journal details
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