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PMID: 6151113 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of mutations that lie in the promoter-regulatory region for glnA, the structural gene encoding glutamine synthetase.

Molecular & general genetics : MGG ·Vol. 197 ·No. 1 ·1984-00-00 ·Pages 150-60

McCarter L, Krajewska-Grynkiewicz K, Trinh D, Wei G, Kustu S

Abstract

In enteric bacteria products of nitrogen regulatory genes ntrA, ntrB and ntrC are known to regulate transcription both positively and negatively at glnA, the structural gene encoding glutamine synthetase [L-glutamate:ammonia-ligase (ADP-forming), EC 6.3.1.2]. We have characterized two types of cis-acting mutations in the glnA promoter-regulatory region. One type, which we have called promoter Up [glnAp (Up)], elevates transcription of glnA to high levels without need for ntr-mediated activation but leaves expression sensitive to ntr-mediated repression. The other type renders glnA transcription insensitive to repression but leaves it normally responsive to activation. Properties of the two types of promoter-regulatory mutations suggest that sites for ntr-mediated activation of glnA transcription are functionally distinct from sites for ntr-mediated repression.

MeSH Terms
Bacterial Proteins/genetics Gene Expression Regulation Genes, Bacterial Genes, Regulator Glutamate-Ammonia Ligase/genetics Operon Promoter Regions, Genetic Repressor Proteins/genetics Salmonella typhimurium/genetics
Chemicals
Bacterial Proteins Repressor Proteins Glutamate-Ammonia Ligase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
McCarter L
Krajewska-Grynkiewicz K
Trinh D
Wei G
Kustu S
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40 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327936
Published
1984-00-00
Pages
150-60
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM21307 · United States
External Links
PubMed source DOI: 10.1007/BF00327936
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