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PMID: 3882668 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mutational analysis of the Klebsiella pneumoniae nitrogenase promoter: sequences essential for positive control by nifA and ntrC (glnG) products.

Journal of bacteriology ·Vol. 161 ·No. 3 ·1985-03-00 ·Pages 868-74

Ow DW, Xiong Y, Gu Q, Shen SC

Abstract

ntr (nitrogen regulated) and nif (nitrogen fixation) promoters are structurally similar to each other but bear no resemblance to canonic Escherichia coli promoters. ntr promoters are normally activated by the ntrC (glnG) product, but they can also be activated by the ntrC-related Klebsiella pneumoniae nifA product. In contrast, nif promoters of K. pneumoniae such as the nitrogenase (nifH) promoter can only be nifA activated. In this paper, we report the isolation and characterization of 28 mutants of the K. pneumoniae nifH promoter. Class A mutants no longer respond to nifA-mediated transcription, and class B mutants can now respond to ntrC-mediated activation. These two classes of mutants define sequences important to nifA- and ntrC-mediated transcription. Most surprising is that a single base change is sufficient to convert a nifA-activated promoter into an ntrC-activated one.

MeSH Terms
Base Sequence Gene Expression Regulation Klebsiella pneumoniae/enzymology,genetics Mutation Nitrogenase/genetics Operon Promoter Regions, Genetic Transcription, Genetic
Chemicals
Nitrogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ow D W
Xiong Y
Gu Q
Shen S C
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.3.868-874.1985
Published
1985-03-00
Pages
868-74
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214977
Subset
IM
Databases
GENBANK
M10171, M10172
External Links
PubMed source DOI: 10.1128/jb.161.3.868-874.1985 PMC Full Text View journal details
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