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PMID: 7000753 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mutations in nif genes that cause Klebsiella pneumoniae to be derepressed for nitrogenase synthesis in the presence of ammonium.

Journal of bacteriology ·Vol. 144 ·No. 2 ·1980-11-00 ·Pages 744-51

MacNeil D, Brill WJ

Abstract

Four Nif+ revertants from strains with polar insertions in nifL, were insensitive to ammonium and amino acid repression of nitrogenase synthesis. These strains have mutations located in or near the nifL region. The derepressed phenotype was dominant in a merodiploid containing a nif+ plasmid. These nif regulatory mutations also suppressed the Nif- phenotype of Gln- strains. Thus, regulation by fixed nitrogen (possible via glutamine synthetase) occurs on the nifLA operon but not on the other six nif operons.

MeSH Terms
Gene Expression Regulation Genes, Regulator Klebsiella pneumoniae/enzymology,genetics Mutation Nitrogenase/biosynthesis Operon Quaternary Ammonium Compounds/pharmacology
Chemicals
Quaternary Ammonium Compounds Nitrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
MacNeil D
Brill W J
References (23)
23 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.144.2.744-751.1980
Published
1980-11-00
Pages
744-51
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294724
Subset
IM
Grants
NIGMS NIH HHS · GM22130 · United States
External Links
PubMed source DOI: 10.1128/jb.144.2.744-751.1980 PMC Full Text View journal details
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