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PMID: 362160 Published · ppublish English Journal Article

Polarity of mutations induced by insertion of transposons Tn5, Tn7 and Tn10 into the nif gene cluster of Klebsiella pneumoniae.

Molecular & general genetics : MGG ·Vol. 165 ·No. 1 ·1978-09-20 ·Pages 103-11

Merrick M, Filser M, Kennedy C, Dixon R

Abstract

Three new genes nifM, nifI and nifN have been mapped in the nif gene cluster of Klebsiella pneumoniae and a fourth gene nifJ has been confirmed as being a separate cistron. Polar nif mutations were obtained by transposition of Tn7 to plasmid pRD1, and of Tn5 and Tn10 to plasmid pMF100, a derivative of pRD1. Complementation analysis of the nif::Tn mutants led to the identification of at least six transcriptional units: nifB; nifA; nifJ; nifH, nifD and nifK; nifE and nifI; nifN, nifM and nifF. Biochemical and genetic evidence suggest that the three genes nifH, nifD and nifK, which are probably the structural genes for nitrogenase, belong to the same operon and are transcribed from nifH to nifK. A polypeptide with a molecular weight of approximately 120,000 is presumed to be the nifJ product.

MeSH Terms
Chromosome Mapping Chromosomes, Bacterial Genes Klebsiella pneumoniae/genetics Nitrogenase/genetics Transcription, Genetic Transformation, Bacterial
Chemicals
Nitrogenase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Merrick M
Filser M
Kennedy C
Dixon R
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23 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00270382
Published
1978-09-20
Pages
103-11
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00270382
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