Klebsiella pneumoniae M5a1 grows well in the presence or absence of molybdenum in media containing excess NH(4) (+). However, growth on N(2) is completely dependent on the presence of molybdenum in the medium. Tungstate competes with the molybdate requirement during growth on N(2). In molybdenum-depleted medium, neither protein component of nitrogenase is active and neither component can be detected antigenically. These data provide evidence that molybdenum is an inducer of nitrogenase synthesis.
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