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PMID: 6149519 Published · ppublish English Comparative Study Journal Article

Tandem promoters determine regulation of the Klebsiella pneumoniae glutamine synthetase (glnA) gene.

Nucleic acids research ·Vol. 12 ·No. 20 ·1984-10-25 ·Pages 7811-30

Dixon R

Abstract

Transcription of the structural gene for glutamine synthetase (glnA) in Klebsiella pneumoniae is controlled by the nitrogen regulatory genes ntrA, ntrB and ntrC. The nucleotide sequence of the regulatory region upstream of the glnA gene is reported here. High resolution S1 mapping of in vivo transcripts indicates that the regulatory region contains tandem promoters separated by 100 nucleotides. Measurements of beta-galactosidase activities determined in vivo from glnA-lac fusions suggest that the upstream promoter (for RNA2) is negatively regulated by the ntrBC gene products whereas transcription from the downstream promoter (for RNA1) is positively activated by the ntrA gene product in the presence of either the ntrBC or the nifA genes. The nucleotide sequence of the upstream promoter resembles the consensus sequence for E. coli promoters, whereas the downstream promoter shows homology with the nitrogen fixation (nif) promoters of K. pneumoniae.

MeSH Terms
Base Sequence Cloning, Molecular DNA Restriction Enzymes Gene Expression Regulation Genes Genes, Bacterial Genes, Regulator Glutamate-Ammonia Ligase/genetics Klebsiella pneumoniae/enzymology,genetics Plasmids Promoter Regions, Genetic Species Specificity Transcription, Genetic beta-Galactosidase/genetics
Chemicals
DNA Restriction Enzymes beta-Galactosidase Glutamate-Ammonia Ligase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Dixon R
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41 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.20.7811
Published
1984-10-25
Pages
7811-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320202
Subset
IM
Databases
GENBANK
X01085
External Links
PubMed source DOI: 10.1093/nar/12.20.7811 PMC Full Text View journal details
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