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PMID: 3099165 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Product of Saccharomyces cerevisiae nuclear gene PET494 activates translation of a specific mitochondrial mRNA.

Molecular and cellular biology ·Vol. 6 ·No. 11 ·1986-11-00 ·Pages 3694-703

Costanzo MC, Fox TD

Abstract

The product of Saccharomyces cerevisiae nuclear gene PET494 is known to be required for a posttranscriptional step in the accumulation of one mitochondrial gene product, subunit III of cytochrome c oxidase (coxIII). Here we show that the PET494 protein probably acts in mitochondria by demonstrating that both a PET494-beta-galactosidase fusion protein and unmodified PET494 are specifically associated with mitochondria. To define the PET494 site of action, we isolated mutations that suppress a pet494 deletion. These mutations were rearrangements of the mitochondrial gene oxi2 that encodes coxIII. The suppressor oxi2 genes had acquired the 5'-flanking sequences of other mitochondrial genes and gave rise to oxi2 transcripts carrying the 5'-untranslated leaders of their mRNAs. These results demonstrate that in wild-type cells PET494 specifically promotes coxIII translation, probably by interacting with the 5'-untranslated leader of the oxi2 mRNA.

MeSH Terms
Base Sequence Cell Nucleus/metabolism Genes Genes, Fungal Mutation Plasmids Protein Biosynthesis RNA Processing, Post-Transcriptional RNA, Messenger/genetics RNA, Mitochondrial Saccharomyces cerevisiae/genetics beta-Galactosidase/genetics
Chemicals
RNA, Messenger RNA, Mitochondrial mitochondrial messenger RNA beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Costanzo M C
Fox T D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.11.3694-3703.1986
Published
1986-11-00
Pages
3694-703
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367130
Subset
IM
Grants
NIGMS NIH HHS · GM29362 · United States
NICHD NIH HHS · HD00515 · United States
Databases
GENBANK
J01478
External Links
PubMed source DOI: 10.1128/mcb.6.11.3694-3703.1986 PMC Full Text View journal details
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