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PMID: 3916733 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mitochondrial suppression of a yeast nuclear mutation which affects the translation of the mitochondrial apocytochrome b transcript.

Current genetics ·Vol. 9 ·No. 8 ·1985-00-00 ·Pages 641-8

Rödel G, Körte A, Kaudewitz F

Abstract

We describe a mitochondrial suppressor mutation, which restores respiratory competence to the nuclear pet- -mutant MK2. This mutant lacks the message of the mitochondrial cob-gene and instead accumulates a partially spliced pre-mRNA which is not translated. Complete processing and translation of the cob-RNA is restored by a rearrangement of the mitochondrial DNA, leading to a fusion of the cob-coding sequences with the leader of oli1, the mitochondrial gene coding for subunit IX of the ATPase. We conclude that the nuclear gene affected in MK2 is essential to allow translation of transcripts which contain the cob-leader sequence.

MeSH Terms
Apoproteins/genetics Base Sequence Cell Nucleus/metabolism Cytochrome b Group/genetics Cytochromes b Genes Genes, Fungal Mitochondria/metabolism Molecular Sequence Data Mutation Protein Biosynthesis Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
Apoproteins Cytochrome b Group Cytochromes b
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rödel G
Institut für Genetik und Mikrobiologie, Ludwig-Maximilians-Universität, München, Federal Republic of Germany.
Körte A
Kaudewitz F
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00449816
Published
1985-00-00
Pages
641-8
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00449816 View journal details
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