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PMID: 6223276 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Biogenesis of mitochondria: the mitochondrial gene (aap1) coding for mitochondrial ATPase subunit 8 in Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 11 ·No. 13 ·1983-07-11 ·Pages 4435-51

Macreadie IG, Novitski CE, Maxwell RJ, John U, Ooi BG, McMullen GL, Lukins HB, Linnane AW, Nagley P

Abstract

A mitochondrial gene (denoted aap1) in Saccharomyces cerevisiae has been characterized by nucleotide sequence analysis of a region of mtDNA between the oxi3 and oli2 genes. The reading frame of the aap1 gene specifies a hydrophobic polypeptide containing 48 amino acids. The functional nature of this reading frame was established by sequence analysis of a series of mit- mutants and revertants. Evidence is presented that the aap1 gene codes for a mitochondrially synthesized polypeptide associated with the mitochondrial ATPase complex. This polypeptide (denoted subunit 8) is a proteolipid whose size has been previously assumed to be 10 kilodaltons based on its mobility on SDS-polyacrylamide gels, but the sequence of the aap1 gene predicts a molecular weight of 5,815 for this protein.

MeSH Terms
Adenosine Triphosphatases/genetics Amino Acid Sequence Base Sequence DNA, Mitochondrial/genetics Genes Mitochondria/enzymology Molecular Weight Mutation Saccharomyces cerevisiae/enzymology,genetics
Chemicals
DNA, Mitochondrial Adenosine Triphosphatases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Macreadie I G
Novitski C E
Maxwell R J
John U
Ooi B G
McMullen G L
Lukins H B
Linnane A W
Nagley P
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.13.4435
Published
1983-07-11
Pages
4435-51
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326057
Subset
IM
Databases
GENBANK
K00035
External Links
PubMed source DOI: 10.1093/nar/11.13.4435 PMC Full Text View journal details
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