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PMID: 6387398 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Organization and processing of the mitochondrial oxi3/oli2 multigenic transcript in yeast.

Molecular & general genetics : MGG ·Vol. 196 ·No. 2 ·1984-00-00 ·Pages 266-74

Simon M, Faye G

Abstract

In the present article, we confirm our previous proposal (Faye and Simon 1983a, b) that the oxi3 and oli2 genes belong to the same transcription unit. Furthermore, we have shown that a primary polycistronic transcript covers oxi3, aap1, oli2 and extends beyond URF2. Transcriptional analysis of this region revealed several cleavage points. The examination of the DNA sequence at and surrounding these cleavage points disclosed that some of them take place at or near specific sequences found also in other known multigenic transcripts. Two of the major cleavages involve the stem-loop structure of GC rich clusters. We discuss the possibility that some of these cleavage sites serve as post-transcriptional processing signals and may be necessary for the maturation of the precursor RNA.

MeSH Terms
Base Sequence DNA, Fungal/genetics DNA, Mitochondrial/genetics Genes, Fungal Nucleic Acid Conformation RNA Processing, Post-Transcriptional RNA, Fungal/metabolism RNA, Messenger/metabolism Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
DNA, Fungal DNA, Mitochondrial RNA, Fungal RNA, Messenger
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Simon M
Faye G
References (43)
43 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00328059
Published
1984-00-00
Pages
266-74
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X00960
External Links
PubMed source DOI: 10.1007/BF00328059
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