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PMID: 6760126 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcripts of mitochondrial tRNA genes in Saccharomyces cerevisiae.

Nucleic acids research ·Vol. 10 ·No. 22 ·1982-11-25 ·Pages 7283-93

Frontali L, Palleschi C, Francisci S

Abstract

The transcription of a group of tRNA genes from the large tRNA gene cluster of mitochondrial DNA from Saccharomyces cerevisiae has been investigated by hybridization with DNA probes carrying tRNA coding sequences and small portions of the A + T rich intergenic regions. Results have shown that in some rho- mutants (DS502, F11) mature tRNA was absent, but a few transcripts could be detected. Some high molecular weight species actually hybridized with DNA probes carrying different tRNA coding sequences. Low molecular weight transcripts (100-150 nucleotides, carrying one tRNA sequence) were also present in these mutants. A high molecular weight transcript was also observed in the wild type, though in much more limited amount. The low molecular weight transcripts were analysed by the S1 mapping technique and found to include both a tRNA sequence and the upstream 5' flanking region extending as far as the 3' end of the preceding tRNA gene. The results suggest the existence of a common transcript bearing several tRNA sequences and indicate a possible mechanism of processing, which might be defective in mutants.

MeSH Terms
Base Sequence DNA, Mitochondrial/genetics Genes Mitochondria/metabolism Molecular Weight Mutation Nucleic Acid Hybridization RNA, Transfer/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
DNA, Mitochondrial RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Frontali L
Palleschi C
Francisci S
References (27)
27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.22.7283
Published
1982-11-25
Pages
7283-93
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327004
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.22.7283 PMC Full Text View journal details
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