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PMID: 7003547 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Transfer RNA genes in the cap-oxil region of yeast mitochondrial DNA.

Nucleic acids research ·Vol. 8 ·No. 21 ·1980-11-11 ·Pages 5017-30

Berlani RE, Bonitz SG, Coruzzi G, Nobrega M, Tzagoloff A

Abstract

A cytoplasmic "petite" (rho-) clone of Saccharomyces cerevisiae has been isolated and found through DNA sequencing to contain the genes for cysteine, histidine, leucine, glutamine, lysine, arginine, and glycine tRNAs. This clone, designated DS502, has a tandemly repeated 3.5 kb segment of the wild type genome from 0.7 to 5.6 units. All the tRNA genes are transcribed from the same strand of DNA in the direction cap to oxil. The mitochondrial DNA segment of DS502 fills a sequence gap that existed between the histidine and lysine tRNAs. The new sequence data has made it possible to assign accurate map positions to all the tRNA genes in the cap-oxil span of the yeast mitochondrial genome. A detailed restriction map of the region from 0 to 17 map units along with the locations of 16 tRNA genes have been determined. The secondary structures of the leucine and glutamine tRNAs have been deduced from their gene sequences. The leucine tRNA exhibits 64% sequence homology to an E. coli leucine tRNA.

MeSH Terms
Base Sequence Chromosome Mapping DNA, Fungal/genetics DNA, Mitochondrial/genetics Genes Mutation Nucleic Acid Conformation RNA, Fungal/genetics RNA, Transfer/genetics Saccharomyces cerevisiae/genetics
Chemicals
DNA, Fungal DNA, Mitochondrial RNA, Fungal RNA, Transfer
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Berlani R E
Bonitz S G
Coruzzi G
Nobrega M
Tzagoloff A
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.21.5017
Published
1980-11-11
Pages
5017-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324276
Subset
IM
Databases
GENBANK
K00538, K00539, L00011, L00012, V00693
External Links
PubMed source DOI: 10.1093/nar/8.21.5017 PMC Full Text View journal details
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