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PMID: 6092852 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning and genetic mapping of the PET494 gene of Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 195 ·No. 1-2 ·1984-00-00 ·Pages 275-80

Müller PP, Fox TD

Abstract

The activity of the nuclear gene PET494 is required to allow expression of the yeast mitochondrial gene oxi2. To aid the study of the mechanism of action of PET494 we have isolated this gene from yeast DNA. A clone bank of yeast DNA fragments in a yeast-E. coli shuttle vector was screened by transformation for a plasmid able to complement the pet494-1 amber mutation. A complementing plasmid was obtained that contained a unique 4.4 kb yeast sequence. This 4.4 kb sequence contains the PET494 gene. Integration of a plasmid containing it into chromosomal DNA by homologous recombination, and subsequent genetic analysis, demonstrated that the 4.4 kb fragment was tightly linked to the pet494-1 mutation. In addition, the corresponding 4.4 kb sequence isolated from a pet494-1 mutant failed to complement the mutation. A 2 kb fragment, subcloned from the original plasmid retained the ability to complement the mutation. The pet494-1 mutation maps to chromosome XIV between rna2 and lys9, approximately 2.4 cm from lys9.

MeSH Terms
Base Sequence Cloning, Molecular Crosses, Genetic DNA Restriction Enzymes DNA, Mitochondrial/genetics Genes, Fungal Genotype Mutation Plasmids Saccharomyces cerevisiae/genetics Species Specificity
Chemicals
DNA, Mitochondrial DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Müller P P
Fox T D
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29 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332759
Published
1984-00-00
Pages
275-80
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM29362 · United States
NICHD NIH HHS · HD00515 · United States
External Links
PubMed source DOI: 10.1007/BF00332759
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