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PMID: 6329697 Published · ppublish English Journal Article

Two yeast acid phosphatase structural genes are the result of a tandem duplication and show different degrees of homology in their promoter and coding sequences.

The EMBO journal ·Vol. 1 ·No. 6 ·1982-00-00 ·Pages 675-80

Meyhack B, Bajwa W, Rudolph H, Hinnen A

Abstract

We have cloned the structural genes for a regulated ( PHO5 ) and a constitutive ( PHO3 ) acid phosphatase from yeast by transformation and complementation of a yeast pho3 , pho5 double mutant. Both genes are located on a 5.1-kb BamHI fragment. The cloned genes were identified on the basis of genetic evidence and by hybrid selection of mRNA coupled with in vitro translation and immunoprecipitation. Subcloning of partial Sau3A digests and functional in vivo analysis by transformation together with DNA sequence analysis showed that the two genes are oriented in the order (5') PHO5 , PHO3 (3'). While the nucleotide sequences of the two coding regions are quite similar, the putative promoter regions show a lower degree of sequence homology. Partly divergent promoter sequences may explain the different regulation of the two genes.

MeSH Terms
Acid Phosphatase/genetics Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes Genes, Fungal Genetic Complementation Test Mutation Operon Plasmids Saccharomyces cerevisiae/enzymology,genetics
Chemicals
DNA Restriction Enzymes Acid Phosphatase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Meyhack B
Bajwa W
Rudolph H
Hinnen A
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24 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
675-80
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553267
Subset
IM
Databases
GENBANK
M24178
External Links
PubMed source PMC Full Text View journal details
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