Toxic accumulation of alpha-ketobutyrate caused by inhibition of the branched-chain amino acid biosynthetic enzyme acetolactate synthase in Salmonella typhimurium.

Journal of bacteriology ·Vol. 169 ·No. 4 ·1987-04-00 ·Pages 1372-8

LaRossa RA, Van Dyk TK, Smulski DR

Abstract

Biochemical and genetic analyses of the bacterium Salmonella typhimurium suggest that accumulation of alpha-ketobutyrate partially mediates the herbicidal activity of acetolactate synthase inhibitors. Growth inhibition of wild-type bacteria by the herbicide sulfometuron methyl was prevented by supplementing the medium with isoleucine, an allosteric inhibitor of threonine deaminase-catalyzed synthesis of alpha-ketobutyrate. In contrast, isoleucine did not rescue the growth of a mutant containing a threonine deaminase unresponsive to isoleucine. Moreover, the hypersensitivity of seven Tn10 insertion mutants to growth inhibition by sulfometuron methyl and alpha-ketobutyrate correlated with their inability to convert alpha-ketobutyrate to less noxious metabolites. We propose that alpha-ketobutyrate accumulation is an important component of sulfonylurea and imidazolinone herbicide action.

MeSH Terms

Acetolactate Synthase/antagonists & inhibitors Butyrates/metabolism DNA Transposable Elements Isoleucine/pharmacology Kinetics Mutation Oxo-Acid-Lyases/antagonists & inhibitors Salmonella typhimurium/drug effects,enzymology,genetics,metabolism Sulfonylurea Compounds/pharmacology Valine/pharmacology

Chemicals

Butyrates DNA Transposable Elements Sulfonylurea Compounds Isoleucine alpha-ketobutyric acid Acetolactate Synthase Oxo-Acid-Lyases Valine sulfometuron methyl

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

LaRossa R A

Van Dyk T K

Smulski D R

References (24)

24 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.169.4.1372-1378.1987

Published

1987-04-00

Pages

1372-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC211955

Subset

External Links

PubMed source DOI: 10.1128/jb.169.4.1372-1378.1987 PMC Full Text View journal details

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Toxic accumulation of alpha-ketobutyrate caused by inhibition of the branched-chain amino acid biosynthetic enzyme acetolactate synthase in Salmonella typhimurium.

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