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PMID: 7007356 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Organization and regulation of the ilvGEDA operon in Salmonella typhimurium LT2.

Journal of bacteriology ·Vol. 145 ·No. 2 ·1981-02-00 ·Pages 984-9

Berg CM, Shaw KJ

Abstract

A total of 102 isoleucine- and isoleucine-valine-requiring (ilv) mutants induced by insertion of the transposable element Tn10 have been classified to cistron by growth requirement, cross-feeding behavior, and enzyme assays. The mutations are in a polycistronic operon transcribed in the order ilvGEDA and in a monocistronic operon ilvC. Analysis of distal gene expression in these polar insertion mutants revealed the existence of two constitutive interval promoters, one preceding ilvE and the other preceding ilvD.

MeSH Terms
Chromosome Mapping Gene Expression Regulation Genes Isoleucine/genetics Mutation Operon Salmonella typhimurium/genetics Valine/genetics
Chemicals
Isoleucine Valine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Berg C M
Shaw K J
References (15)
15 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.145.2.984-989.1981
Published
1981-02-00
Pages
984-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217208
Subset
IM
Grants
NIGMS NIH HHS · 1T32 GM07584 · United States
NIAID NIH HHS · AI-41278 · United States
External Links
PubMed source DOI: 10.1128/jb.145.2.984-989.1981 PMC Full Text View journal details
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