Home LiteratureArticle Details
PMID: 6245063 Published · ppublish English Journal Article

Salmonella typhimurium mutants defective in acetohydroxy acid synthases I and II.

Journal of bacteriology ·Vol. 141 ·No. 3 ·1980-03-00 ·Pages 1258-63

Shaw KJ, Berg CM, Sobol TJ

Abstract

An analysis of transposon-induced mutants shows that Salmonella typhimurium possesses two major isozymes of acetohydroxy acid synthase, the enzymes which mediate the first common step in isoleucine and valine biosynthesis. A third (minor) acetohydroxy acid synthase is present, but its significance in isoleucine and valine synthesis may be negligible. Mutants defective in acetohydroxy acid synthase II (ilvG::Tn10) require isoleucine, alpha-ketobutyrate, or threonine for growth, a mutant defective in acetohydroxy acid synthase I (ilvB::Tn5) is a prototroph, and a double mutant (ilvG::Tn10 ilvB::Tn5) requires isoleucine plus valine for growth.

MeSH Terms
Acetolactate Synthase/genetics,metabolism DNA Transposable Elements Genes Isoleucine/biosynthesis Mutation Oxo-Acid-Lyases/metabolism Salmonella typhimurium/enzymology,genetics Transduction, Genetic Valine/biosynthesis,pharmacology
Chemicals
DNA Transposable Elements Isoleucine Acetolactate Synthase Oxo-Acid-Lyases Valine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shaw K J
Berg C M
Sobol T J
References (15)
15 references, click to expand
  1. Structural genes for a newly recognized acetolactate synthase in Escherichia coli K-12.
    J Bacteriol. 1974 Dec;120(3):1068-77 PMID: 4612003
  2. Expression of a valine-resistant acetolactate synthase activity mediated by the ilv O and ilv G genes of Escherichia coli K-12.
    Mol Gen Genet. 1976 Feb 2;143(3):243-52 PMID: 765758
  3. Mutations affecting the formation of acetohydroxy acid synthase II in Escherichia coli K-12.
    Mol Gen Genet. 1979 Feb 1;169(3):299-314 PMID: 372752
  4. New Map Location of ilvO in ESCHERICHIA COLI.
    Genetics. 1976 Jun;83(2):201-25 PMID: 17248710
  5. Mutant of Escherichia coli K-12 missing acetolactate synthase activity.
    J Bacteriol. 1974 Oct;120(1):536-8 PMID: 4608700
  6. Isoleucine and valine metabolism in Escherichia coli. XX. Multiple forms of acetohydroxy acid synthetase.
    Biochem Biophys Res Commun. 1972 Jul 25;48(2):444-50 PMID: 4557732
  7. Temperature-sensitive growth inhibition by valine in Salmonella typhimurium: alteration of one form of acetohydroxy acid synthetase.
    J Bacteriol. 1973 Oct;116(1):98-106 PMID: 4583227
  8. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. 3. MAP ORDER OF THE STRUCTURAL GENES AND OPERATOR GENES.
    J Bacteriol. 1965 Mar;89:661-4 PMID: 14273641
  9. Physiological characterization of polar Tn5-induced isoleucine-valine auxotrophs in Escherichia coli K.12: evidence for an internal promoter in the ilvOGEDA operon.
    Genetics. 1979 Oct;93(2):308-19 PMID: 395023
  10. Two forms of biosynthetic acetohydroxy acid synthetase in Salmonella typhimurium.
    Biochem Biophys Res Commun. 1972 Jul 25;48(2):437-43 PMID: 4557731
  11. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
    J Mol Biol. 1977 Oct 15;116(1):125-59 PMID: 338917
  12. Isoleucine-valine requiring mutants of Salmonella typhimurium. 3. Valine-sensitive strains.
    Genetics. 1971 Feb;67(2):171-82 PMID: 4936359
  13. Growth inhibition as a consequence of antagonism between related amino acids: effect of valine in Escherichia coli K-12.
    Microbiol Rev. 1979 Mar;43(1):42-58 PMID: 379577
  14. Deletion mapping of the ilvGOEDAC genes of Escherichia coli K-12.
    Mol Gen Genet. 1979 Feb 1;169(3):289-97 PMID: 372751
  15. Polarity and the regulation of the ilv gene cluster in Escherichia coli strain K-12.
    Mol Gen Genet. 1976 Oct 18;148(2):111-24 PMID: 790150
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.141.3.1258-1263.1980
Published
1980-03-00
Pages
1258-63
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC293821
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.141.3.1258-1263.1980 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2