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PMID: 6997277 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Substrate channeling: alpha-ketobutyrate inhibition of acetohydroxy acid synthase in Salmonella typhimurium.

Journal of bacteriology ·Vol. 143 ·No. 3 ·1980-09-00 ·Pages 1509-12

Shaw KJ, Berg CM

Abstract

Excess alpha-ketobutyrate inhibited the growth of Salmonella typhimurium LT2 by inhibiting the acetohydroxy acid synthase-catalyzed synthesis of alpha-acetolactate (a valine precursor). As a result, cells were starved for valine, and both ilvB (encoding acetohydroxy acid synthase I) and ilvGEDA (ilvG encodes acetohydroxy acid synthase II) were derepressed. The addition of valine reversed the effects of alpha-ketobutyrate.

MeSH Terms
Acetolactate Synthase/antagonists & inhibitors,genetics Butyrates/pharmacology Keto Acids/pharmacology Mutation Oxo-Acid-Lyases/antagonists & inhibitors Salmonella typhimurium/drug effects,enzymology,genetics Valine/pharmacology
Chemicals
Butyrates Keto Acids alpha-ketobutyric acid Acetolactate Synthase Oxo-Acid-Lyases Valine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shaw K J
Berg C M
References (8)
8 references, click to expand
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    Biochem Biophys Res Commun. 1972 Jul 25;48(2):437-43 PMID: 4557731
  2. Isoleucine and valine metabolism in Escherichia coli. XX. Multiple forms of acetohydroxy acid synthetase.
    Biochem Biophys Res Commun. 1972 Jul 25;48(2):444-50 PMID: 4557732
  3. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition.
    J Mol Biol. 1975 Oct 5;97(4):561-75 PMID: 1102715
  4. Mutations affecting the formation of acetohydroxy acid synthase II in Escherichia coli K-12.
    Mol Gen Genet. 1979 Feb 1;169(3):299-314 PMID: 372752
  5. Physiological characterization of polar Tn5-induced isoleucine-valine auxotrophs in Escherichia coli K.12: evidence for an internal promoter in the ilvOGEDA operon.
    Genetics. 1979 Oct;93(2):308-19 PMID: 395023
  6. Salmonella typhimurium mutants defective in acetohydroxy acid synthases I and II.
    J Bacteriol. 1980 Mar;141(3):1258-63 PMID: 6245063
  7. Improved synthesis of the sodium salt of DL-alpha, beta-dihydroxyisovaleric acid.
    Anal Biochem. 1980 May 15;104(2):485-8 PMID: 7004266
  8. Isoleucine and valine metabolism in Escherichia coli. X. The enzymatic formation of acetohydroxybutyrate.
    J Biol Chem. 1961 Sep;236:2486-91 PMID: 13759955
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.143.3.1509-1512.1980
Published
1980-09-00
Pages
1509-12
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC294547
Subset
IM
Grants
PHS HHS · 1T32 · United States
NIAID NIH HHS · AI-14278 · United States
NIGMS NIH HHS · GM07584 · United States
External Links
PubMed source DOI: 10.1128/jb.143.3.1509-1512.1980 PMC Full Text View journal details
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