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PMID: 6348482 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

2-Ketobutyrate: a putative alarmone of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 190 ·No. 3 ·1983-00-00 ·Pages 452-8

Daniel J, Dondon L, Danchin A

Abstract

2-ketobutyrate is synthesized from threonine by threonine deaminase (dehydratase) in E. coli. The effects of 2-ketobutyrate as a regulatory metabolite were studied in vivo. 2-ketobutyrate was shown to inhibit the phosphoenolpyruvate (PEP): sugar phosphotransferase system resulting in aspartate starvation, elevation of ppGpp endogenous pools, and cessation of growth in E. coli grown in glucose and related carbon sources. Accordingly, we propose that 2-ketobutyrate might serve as an alarmone whose concentration precisely governs the shift from anaerobic growth to aerobic growth in E. coli. Such shifts are common phenomena among the Enterobacteriaceae.

MeSH Terms
Anaerobiosis Aspartic Acid/metabolism Butyrates/pharmacology Escherichia coli/drug effects,growth & development,physiology Guanosine Tetraphosphate/metabolism Hydroxy Acids/pharmacology Phosphoenolpyruvate Sugar Phosphotransferase System/antagonists & inhibitors Structure-Activity Relationship
Chemicals
Butyrates Hydroxy Acids Aspartic Acid Guanosine Tetraphosphate vinylglycolic acid alpha-ketobutyric acid Phosphoenolpyruvate Sugar Phosphotransferase System
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Daniel J
Dondon L
Danchin A
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20 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331076
Published
1983-00-00
Pages
452-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331076
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