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PMID: 6247616 Published · ppublish English Journal Article

Serine sensitivity of Escherichia coli K 12: partial characterization of a serine resistnat mutant that is extremely sensitive to 2-ketobutyrate.

Molecular & general genetics : MGG ·Vol. 178 ·No. 1 ·1980-04-00 ·Pages 155-64

Danchin A, Dondon L

Abstract

E. coli wild type bacteria display sensitivity towards serine. A selection medium is described which allows selection of serine resistant mutants. One such mutant is described which presents pleiotropic alterations: it exhibits a thermosensitive growth pattern, alteration in the metabolism of the pppGpp and ppGpp nucleotides, cAMP intracellular level alteration, extreme sensitivity to 2-ketobutyric acid and a defect in the phosphotransferases permeation system. A conjecture explaining these apparently unrelated defects supposes that serine metabolism interferes via phosphoenol pyruvate with a cytoplasmic control of membrane activity (the mutant would be defective in the coupling between membrane and the protein responsible for its cytoplasmic control) and that 2-ketobutyrate is an effector of this activity.

MeSH Terms
Butyrates/pharmacology Culture Media Cyclic AMP/metabolism Drug Resistance, Microbial Escherichia coli/genetics,metabolism Guanosine Pentaphosphate/metabolism Guanosine Tetraphosphate/metabolism Keto Acids/pharmacology Mutation Phenotype Serine/pharmacology
Chemicals
Butyrates Culture Media Keto Acids Guanosine Tetraphosphate Guanosine Pentaphosphate Serine alpha-ketobutyric acid Cyclic AMP
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Danchin A
Dondon L
References (26)
26 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267224
Published
1980-04-00
Pages
155-64
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267224
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