Thin-layer chromatographic methods to isolate 32P-labeled 5-phosphoribosyl-alpha-1-pyrophosphate (PRPP): determination of cellular PRPP pools and assay of PRPP synthetase activity.

Analytical biochemistry ·Vol. 98 ·No. 2 ·1979-10-01 ·Pages 254-63

Jensen KF, Houlberg U, Nygaard P

Abstract

暂无摘要

MeSH Terms

Chromatography, Thin Layer/methods Escherichia coli/enzymology Isotope Labeling/methods Kinetics Pentosephosphates/analysis Phosphoribosyl Pyrophosphate/analysis Phosphorus Radioisotopes Phosphotransferases/analysis Ribose-Phosphate Pyrophosphokinase/analysis Salmonella typhimurium/enzymology

Chemicals

Pentosephosphates Phosphorus Radioisotopes Phosphoribosyl Pyrophosphate Phosphotransferases Ribose-Phosphate Pyrophosphokinase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jensen K F

Houlberg U

Nygaard P

Article Info

Journal

Analytical biochemistry

Abbr.

Anal Biochem

ISSN

0003-2697

DOI

10.1016/0003-2697(79)90138-6

Published

1979-10-01

Pages

254-63

Language

English

Region

United States

NLM ID

0370535

Subset

External Links

PubMed source DOI: 10.1016/0003-2697(79)90138-6 View journal details

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Thin-layer chromatographic methods to isolate 32P-labeled 5-phosphoribosyl-alpha-1-pyrophosphate (PRPP): determination of cellular PRPP pools and assay of PRPP synthetase activity.

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