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PMID: 3020541 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning, characterization, expression, and chromosomal localization of a human ferritin heavy-chain gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 19 ·1986-10-00 ·Pages 7226-30

Hentze MW, Keim S, Papadopoulos P, O'Brien S, Modi W, Drysdale J, Leonard WJ, Harford JB, Klausner RD

Abstract

A genomic phage clone containing a full-length copy of a functional human gene for ferritin heavy chain has been isolated. The gene consists of four exons spanning approximately 3 kilobases and has been localized to chromosome 11. The functionality of the gene was demonstrated by the fact that both transient transfectants and stable transformants of murine fibroblasts actively transcribe human ferritin heavy-chain mRNA.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosomes, Human, Pair 11 Cloning, Molecular DNA Restriction Enzymes Ferritins/genetics Gene Expression Regulation Genes Humans Transcription, Genetic Transfection
Chemicals
Ferritins DNA Restriction Enzymes
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Hentze M W
Keim S
Papadopoulos P
O'Brien S
Modi W
Drysdale J
Leonard W J
Harford J B
Klausner R D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.19.7226
Published
1986-10-00
Pages
7226-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386688
Subset
IM
Grants
NIADDK NIH HHS · AM17775 · United States
Databases
GENBANK
M14211, M14212, M18522
External Links
PubMed source DOI: 10.1073/pnas.83.19.7226 PMC Full Text View journal details
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