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PMID: 1083028 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Novel mechanism for translational control in regulation of ferritin synthesis by iron.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 3 ·1976-03-00 ·Pages 857-61

Zähringer J, Baliga BS, Munro HN

Abstract

Poly(A)-containing RNA was isolated from the polyribosomal and post-ribosomal fractions of the livers of normal and iron-treated rats. These RNA fractions were then translated in a wheat germ system to provide a measure of the amount of ferritin mRNA present in each fraction. Following iron administration, there was a 2-fold increase in the amount of ferritin mRNA in the polyribosomal fraction. This increase was not inhibited by prior treatment of the rats with actinomycin D or cordycepin, suggesting a cytoplasmic control mechanism. In normal rats, the post-ribosomal fraction contained an amount of ferritin mRNA equal to that in the polyribosomes. When iron was administered, this untranslated ferritin mRNA became reduced to negligible quantities, thus accounting for the doubling of the ferritin mRNA content of the polyribosomal fraction. A scheme is proposed in which translation of the ferritin mRNA in the post-ribosomal fraction is prevented by adhering ferritin subunits. Iron administration removes this inhibition of the translation of ferritin mRNA by promoting aggregation of these subunits into ferritin.

MeSH Terms
Animals Cytoplasm/metabolism Dactinomycin/pharmacology Deoxyadenosines/pharmacology Iron/pharmacology Liver/metabolism,ultrastructure Male Models, Biological Polyribosomes/metabolism Protein Biosynthesis/drug effects RNA, Messenger/metabolism RNA, Transfer/metabolism Rats
Chemicals
Deoxyadenosines RNA, Messenger Dactinomycin RNA, Transfer Iron
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zähringer J
Baliga B S
Munro H N
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44 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.3.857
Published
1976-03-00
Pages
857-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336018
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.3.857 PMC Full Text View journal details
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