Home LiteratureArticle Details

PMID: 6323167 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and sequencing of a full length cDNA coding for a human apoferritin H chain: evidence for a multigene family.

The EMBO journal ·Vol. 3 ·No. 1 ·1984-01-00 ·Pages 23-7

Costanzo F, Santoro C, Colantuoni V, Bensi G, Raugei G, Romano V, Cortese R

Abstract

We have cloned a segment of cDNA from human liver coding for an apoferritin subunit, probably an H chain. Sequence comparison with the available protein sequence shows that our clone corresponds to a ferritin subunit present as a minor species in human spleen and placenta, but as major species in HeLa cells. Northern blot analysis shows the existence of only one band of similar size in human liver, HeLa cells, Daudi lymphoma and Hep3B hepatoma cell lines. In contrast, Southern blot analysis provides evidence for a multigene family.

MeSH Terms

Amino Acid Sequence Apoferritins/genetics Base Sequence Cloning, Molecular DNA/metabolism DNA Restriction Enzymes Ferritins/analogs & derivatives Genes Genetic Vectors Humans Liver/metabolism Macromolecular Substances

Chemicals

Macromolecular Substances DNA Ferritins Apoferritins DNA Restriction Enzymes

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Costanzo F

Santoro C

Colantuoni V

Bensi G

Raugei G

Romano V

Cortese R

References (15)

15 references, click to expand

Cloning of several cDNA segments coding for human liver proteins.
EMBO J. 1983;2(1):57-61 PMID: 11894909
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
3' non-coding region sequences in eukaryotic messenger RNA.
Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
Ferritinaemia in cancer.
Nature. 1977 Feb 24;265(5596):755-6 PMID: 67563
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites.
Nature. 1978 Mar 23;272(5651):375-7 PMID: 416358
On ferritin heterogeneity. Further evidence for heteropolymers.
J Biol Chem. 1978 Jun 25;253(12):4451-8 PMID: 659425
Cloning of nematode tRNA genes and their expression in the frog oocyte.
Nucleic Acids Res. 1978 Dec;5(12):4593-611 PMID: 370775
Iron transport and storage proteins.
Annu Rev Biochem. 1980;49:357-93 PMID: 6996567
Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4147-51 PMID: 7001455
The making of strand-specific M13 probes.
Gene. 1982 Mar;17(3):271-7 PMID: 7049837
Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product.
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5661-5 PMID: 6957884
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
Gene. 1982 Oct;19(3):259-68 PMID: 6295879
Isolation of cDNA clones for the light subunit of rat liver ferritin: evidence that the light subunit is encoded by a multigene family.
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1265-9 PMID: 6187009
Serum ferritin concentration in Gaucher's disease.
Br Med J (Clin Res Ed). 1983 Jun 11;286(6381):1864 PMID: 6407607

Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1984-01-00

Pages

23-7

Language

English

Region

England

NLM ID

8208664

PMCID

PMC557292

Subset

Databases

GENBANK

X00318

External Links

PubMed source PMC Full Text View journal details

Analysis Services

Cloning and sequencing of a full length cDNA coding for a human apoferritin H chain: evidence for a multigene family.

Contact

WeChat Official Account