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PMID: 3004206 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A deletion map of the human Y chromosome based on DNA hybridization.

American journal of human genetics ·Vol. 38 ·No. 2 ·1986-02-00 ·Pages 109-24

Vergnaud G, Page DC, Simmler MC, Brown L, Rouyer F, Noel B, Botstein D, de la Chapelle A, Weissenbach J

Abstract

The genomes of 27 individuals (19 XX males, two XX hermaphrodites, and six persons with microscopically detectable anomalies of the Y chromosome) were analyzed by hybridization for the presence or absence of 23 Y-specific DNA restriction fragments. Y-specific DNA was detected in 12 of the XX males and in all six individuals with microscopic anomalies. The results are consistent with each of these individuals carrying a single contiguous portion of the Y chromosome; that is, the results suggest a deletion map of the Y chromosome, in which each of the 23 Y-specific restriction fragments tested can be assigned to one of seven intervals. We have established the polarity of this map with respect to the long and short arms of the Y chromosome. On the short arm, there is a large cluster of sequences homologous to the X chromosome. The testis determinant(s) map to one of the intervals on the short arm.

MeSH Terms
Chromosome Banding Chromosome Deletion Chromosome Mapping DNA/genetics DNA Restriction Enzymes Disorders of Sex Development/genetics Female Genetic Markers Humans Male Nucleic Acid Hybridization Sex Chromosome Aberrations/genetics Y Chromosome
Chemicals
Genetic Markers DNA DNA Restriction Enzymes
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Vergnaud G
Page D C
Simmler M C
Brown L
Rouyer F
Noel B
Botstein D
de la Chapelle A
Weissenbach J
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Article Info
Journal
American journal of human genetics
Abbr.
Am J Hum Genet
ISSN
0002-9297
Published
1986-02-00
Pages
109-24
Language
English
Region
United States
NLM ID
0370475
PMCID
PMC1684749
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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