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PMID: 6689068 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification and isolation of transcribed human X chromosome DNA sequences.

Nucleic acids research ·Vol. 11 ·No. 22 ·1983-11-25 ·Pages 7961-79

Kunkel LM, Tantravahi U, Kurnit DM, Eisenhard M, Bruns GP, Latt SA

Abstract

A human X chromosome specific phage library has been used as a source of X-specific genomic DNA clones which hybridize with cellular RNA. Random cDNA clones were mapped for X chromosome sequence localization and 8 were identified as hybridizing to X chromosome Hind III fragments. All eight also hybridized with autosomal Hind III fragments. The X chromosome genomic sequences corresponding to two of these cDNA clones were isolated from a phage library constructed with the Hind III endonuclease digest products of X enriched DNA. One genomic DNA segment, localized to the short area of the X, shared sequence homology with at least one region of the human Y chromosome. The methodology developed represents a rapid means to obtain a specific genomic DNA clone from a single chromosome when multiple different genomic loci homologous to an expressed DNA sequence exist.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/metabolism Female HeLa Cells/physiology Humans Hybrid Cells/physiology Male Nucleic Acid Hybridization Plasmids Transcription, Genetic X Chromosome/physiology Y Chromosome/physiology
Chemicals
DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kunkel L M
Tantravahi U
Kurnit D M
Eisenhard M
Bruns G P
Latt S A
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.22.7961
Published
1983-11-25
Pages
7961-79
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326552
Subset
IM
Grants
NIGMS NIH HHS · GM21121/33579 · United States
NICHD NIH HHS · HD04807 · United States
NICHD NIH HHS · K08HD00453 · United States
External Links
PubMed source DOI: 10.1093/nar/11.22.7961 PMC Full Text View journal details
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