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PMID: 6092051 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cosmid clones derived from both euchromatic and heterochromatic regions of the human Y chromosome.

The EMBO journal ·Vol. 3 ·No. 9 ·1984-09-00 ·Pages 1997-2003

Wolfe J, Erickson RP, Rigby PW, Goodfellow PN

Abstract

Clones containing sequences derived from the human Y chromosome have been isolated from cosmid libraries of a human-mouse hybrid cell line. These libraries were constructed in the new expression vectors Homer V and Homer VI. The collection of cosmids isolated is enriched for unique sequence DNA and only a few of the cosmids contain the tandemly repeated sequences which constitute a major portion of the Y chromosome. Three cosmids have been studied in detail. One cosmid shows extensive homology over at least 20 kb with the long arm of the X chromosome; this homology is outside the predicted homology region required for sex chromosome pairing. The other two clones contain unique sequences specific to the Y chromosome and both map to the heterochromatic region of the Y chromosome long arm.

MeSH Terms
Animals Base Sequence Chromatin/physiology Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes Heterochromatin/physiology Humans Hybrid Cells/cytology Male Mice Plasmids Y Chromosome
Chemicals
Chromatin Heterochromatin DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wolfe J
Erickson R P
Rigby P W
Goodfellow P N
References (36)
36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-09-00
Pages
1997-2003
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557633
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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