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PMID: 2999430 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two independent mutations are required for temperature-sensitive cell transformation by a Rous sarcoma virus temperature-sensitive mutant.

Journal of virology ·Vol. 56 ·No. 3 ·1985-12-00 ·Pages 743-9

Nishizawa M, Mayer BJ, Takeya T, Yamamoto T, Toyoshima K, Hanafusa H, Kawai S

Abstract

We molecularly cloned the src coding region of tsNY68, a mutant of Rous sarcoma virus temperature sensitive (ts) for transformation, and constructed a series of ts wild-type recombinant src genes. DNA containing the hybrid genes was transfected into chicken cells together with viral vector DNA and helper viral DNA, and infectious transforming viruses were recovered. Characterization of these recombinant viruses indicated that at least two mutations are present in the 3' half of the mutant src gene, both of which are required for ts. Nucleotide sequence analysis revealed three differences in the deduced amino acid sequence compared with the parental virus. Two of these changes, a deletion of amino acids 352 to 354 and an amino acid substitution at position 461, are responsible for the ts phenotype.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics Base Sequence Cell Transformation, Viral Cells, Cultured Chick Embryo Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes Genes, Viral Mutation Oncogenes Temperature
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Nishizawa M
Mayer B J
Takeya T
Yamamoto T
Toyoshima K
Hanafusa H
Kawai S
References (48)
48 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.56.3.743-749.1985
Published
1985-12-00
Pages
743-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252644
Subset
IM
Grants
NCI NIH HHS · CA14935 · United States
Databases
GENBANK
M11753
External Links
PubMed source DOI: 10.1128/JVI.56.3.743-749.1985 PMC Full Text View journal details
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