Home LiteratureArticle Details
PMID: 2901725 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of DNA sequences required for mouse APRT gene expression.

Nucleic acids research ·Vol. 16 ·No. 17 ·1988-09-12 ·Pages 8509-24

Dush MK, Briggs MR, Royce ME, Schaff DA, Khan SA, Tischfield JA, Stambrook PJ

Abstract

The mouse aprt promoter contains four GC boxes, which bind transcription factor Spl in vitro, and lacks both TATA and CCAAT boxes. Removal of the two most distal GC boxes of this promoter had little effect on APRT enzyme levels produced in a transient expression assay. Deletion of the distal three GC boxes resulted in a 50% reduction, and deletion of all GC boxes resulted in essentially complete loss of APRT activity. There are two predominant transcription start sites which are located within the region containing the GC boxes. The promoter behaved as a relatively strong promoter when compared to the RSV LTR promoter in a transient CAT assay, and operated in one orientation only. No upstream anti-sense transcripts were detected in either mouse CAK or liver cells, confirming that the mouse aprt promoter, unlike some other GC-rich promoters appears not to support bidirectional transcription.

MeSH Terms
Adenine Phosphoribosyltransferase/genetics,metabolism Animals Base Sequence Cell Line Chromosome Deletion Genes Genes, Homeobox Kinetics Mice Molecular Sequence Data Mutation Pentosyltransferases/genetics Plasmids Promoter Regions, Genetic Transcription, Genetic Transfection
Chemicals
Pentosyltransferases Adenine Phosphoribosyltransferase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Dush M K
Department of Anatomy and Cell Biology, University of Cincinnati College of Medicine, OH 45267.
Briggs M R
Royce M E
Schaff D A
Khan S A
Tischfield J A
Stambrook P J
References (61)
61 references, click to expand
  1. Human dihydrofolate reductase gene organization. Extensive conservation of the G + C-rich 5' non-coding sequence and strong intron size divergence from homologous mammalian genes.
    J Mol Biol. 1984 Jun 25;176(2):169-87 PMID: 6235374
  2. HMG CoA reductase: a negatively regulated gene with unusual promoter and 5' untranslated regions.
    Cell. 1984 Aug;38(1):275-85 PMID: 6088070
  3. A sequence downstream of AAUAAA is required for rabbit beta-globin mRNA 3'-end formation.
    Nature. 1984 Nov 29-Dec 5;312(5993):473-4 PMID: 6095108
  4. Heterogeneity at the 5' termini of mouse dihydrofolate reductase mRNAs. Evidence for multiple promoter regions.
    J Biol Chem. 1985 Feb 25;260(4):2307-14 PMID: 2982814
  5. Sequence of the promoter region of the gene for human X-linked 3-phosphoglycerate kinase.
    Gene. 1984 Dec;32(3):409-17 PMID: 6099325
  6. Nucleotide sequence of hamster adenine phosphoribosyl transferase (aprt) gene.
    Nucleic Acids Res. 1986 Feb 25;14(4):1914 PMID: 3952002
  7. A re-examination of the 5' termini of mouse dihydrofolate reductase RNA.
    J Biol Chem. 1986 Apr 5;261(10):4685-90 PMID: 3007471
  8. Fine structure genetic analysis of a beta-globin promoter.
    Science. 1986 May 2;232(4750):613-8 PMID: 3457470
  9. Transcriptional "silencer" element in rat repetitive sequences associated with the rat insulin 1 gene locus.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3151-5 PMID: 3010279
  10. CpG-rich islands and the function of DNA methylation.
    Nature. 1986 May 15-21;321(6067):209-13 PMID: 2423876
  11. Analysis of the mouse dhfr promoter region: existence of a divergently transcribed gene.
    Mol Cell Biol. 1985 Aug;5(8):1847-58 PMID: 3018531
  12. Purification and biochemical characterization of the promoter-specific transcription factor, Sp1.
    Science. 1986 Oct 3;234(4772):47-52 PMID: 3529394
  13. Multiple transcription start sites, DNase I-hypersensitive sites, and an opposite-strand exon in the 5' region of the CHO dhfr gene.
    Mol Cell Biol. 1986 Feb;6(2):425-40 PMID: 3023846
  14. Comparative anatomy of the human APRT gene and enzyme: nucleotide sequence divergence and conservation of a nonrandom CpG dinucleotide arrangement.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3349-53 PMID: 3554238
  15. Transient expression of a mouse alpha-fetoprotein minigene: deletion analyses of promoter function.
    Mol Cell Biol. 1983 Jul;3(7):1295-309 PMID: 6193415
  16. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
    Cell. 1983 Nov;35(1):79-87 PMID: 6313230
  17. Genomic cloning and preliminary characterization of the human thymidine kinase gene.
    Proc Natl Acad Sci U S A. 1983 Nov;80(21):6528-32 PMID: 6314329
  18. cis and trans activation of globin gene transcription in transient assays.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7428-32 PMID: 6200874
  19. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):2147-51 PMID: 6326107
  20. The distal transcription signals of the herpesvirus tk gene share a common hexanucleotide control sequence.
    Cell. 1984 May;37(1):253-62 PMID: 6233005
  21. Requirement for the 3' flanking region of the bovine growth hormone gene for accurate polyadenylylation.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):3944-8 PMID: 6146135
  22. Structural and functional characterization of the promoter region of the mouse c-Ki-ras gene.
    Mol Cell Biol. 1987 Jul;7(7):2592-6 PMID: 3614201
  23. Reversible silencing of enhancers by sequences derived from the human IFN-alpha promoter.
    Cell. 1987 Sep 25;50(7):1057-69 PMID: 3040267
  24. Nucleotide sequence of the human APRT gene.
    Nucleic Acids Res. 1987 Nov 11;15(21):9086 PMID: 3684585
  25. Alternative transcription and splicing of the human porphobilinogen deaminase gene result either in tissue-specific or in housekeeping expression.
    Proc Natl Acad Sci U S A. 1988 Jan;85(1):6-10 PMID: 3422427
  26. Analysis of signals controlling expression of the Chinese hamster ovary aprt gene.
    Mol Cell Biol. 1988 Jun;8(6):2536-44 PMID: 3405212
  27. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  28. The expression of three abundance classes of messenger RNA in mouse tissues.
    Cell. 1976 Dec;9(4 PT 2):761-74 PMID: 1017013
  29. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  30. Purification of adenine phosphoribosyltransferase by affinity chromatography.
    Prep Biochem. 1978;8(6):453-62 PMID: 748918
  31. Transformation of mammalian cells with genes from procaryotes and eucaryotes.
    Cell. 1979 Apr;16(4):777-85 PMID: 222468
  32. Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1432-6 PMID: 6929494
  33. In vivo sequence requirements of the SV40 early promotor region.
    Nature. 1981 Mar 26;290(5804):304-10 PMID: 6259538
  34. Identification of a promoter component involved in positioning the 5' termini of simian virus 40 early mRNAs.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):100-4 PMID: 6264425
  35. Effect of regional DNA methylation on gene expression.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2560-4 PMID: 3857599
  36. Transcription termination within the E1A gene of adenovirus induced by insertion of the mouse beta-major globin terminator element.
    Cell. 1985 Apr;40(4):897-905 PMID: 2580640
  37. Analysis of transcriptional regulatory signals of the HSV thymidine kinase gene: identification of an upstream control region.
    Cell. 1981 Aug;25(2):385-98 PMID: 6269744
  38. Nucleotide sequence and organization of the mouse adenine phosphoribosyltransferase gene: presence of a coding region common to animal and bacterial phosphoribosyltransferases that has a variable intron/exon arrangement.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2731-5 PMID: 3921964
  39. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.
    Plasmid. 1985 Jan;13(1):31-40 PMID: 3991809
  40. The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3' termini.
    Nucleic Acids Res. 1985 Feb 25;13(4):1347-68 PMID: 2987822
  41. Opposite-strand RNAs from the 5' flanking region of the mouse dihydrofolate reductase gene.
    Proc Natl Acad Sci U S A. 1985 Jun;82(12):3978-82 PMID: 2408272
  42. 5' end of HMG CoA reductase gene contains sequences responsible for cholesterol-mediated inhibition of transcription.
    Cell. 1985 Aug;42(1):203-12 PMID: 3860301
  43. Adenosine deaminase: characterization and expression of a gene with a remarkable promoter.
    EMBO J. 1985 Feb;4(2):437-43 PMID: 3839456
  44. Negative regulation of viral enhancers in undifferentiated embryonic stem cells.
    Cell. 1985 Sep;42(2):519-26 PMID: 2992802
  45. Linker scanning mutagenesis of the 5'-flanking region of the mouse beta-major-globin gene: sequence requirements for transcription in erythroid and nonerythroid cells.
    Mol Cell Biol. 1985 Jun;5(6):1498-511 PMID: 3861935
  46. Sp1 binds to promoter sequences and activates herpes simplex virus 'immediate-early' gene transcription in vitro.
    Nature. 1985 Sep 12-18;317(6033):179-82 PMID: 2993923
  47. Bidirectional SV40 transcription mediated by tandem Sp1 binding interactions.
    Science. 1985 Nov 1;230(4725):511-7 PMID: 2996137
  48. Promoter region of the human Harvey ras proto-oncogene: similarity to the EGF receptor proto-oncogene promoter.
    Science. 1985 Dec 20;230(4732):1378-81 PMID: 2999983
  49. Transcription factor Sp1 recognizes a DNA sequence in the mouse dihydrofolate reductase promoter.
    Nature. 1986 Jan 16-22;319(6050):246-8 PMID: 3945313
  50. Point mutation in the TATA box curtails expression of sea urchin H2A histone gene in vivo.
    Nature. 1981 Nov 12;294(5837):178-80 PMID: 6272121
  51. Identification of DNA sequences required for transcription of the human alpha 1-globin gene in a new SV40 host-vector system.
    Cell. 1981 Dec;27(2 Pt 1):279-88 PMID: 6277501
  52. Transcriptional control signals of a eukaryotic protein-coding gene.
    Science. 1982 Jul 23;217(4557):316-24 PMID: 6283634
  53. Genetic instability at the adenine phosphoribosyltransferase locus in mouse L cells.
    Mol Cell Biol. 1982 Mar;2(3):250-7 PMID: 7110133
  54. Differences in human alpha-, beta- and delta-globin gene expression in monkey kidney cells.
    Cell. 1982 Aug;30(1):173-83 PMID: 6290076
  55. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  56. Deletion mapping of DNA regions required for SV40 early region promoter function in vivo.
    J Mol Appl Genet. 1982;1(5):457-81 PMID: 6296253
  57. Functional relationships between transcriptional control signals of the thymidine kinase gene of herpes simplex virus.
    Cell. 1982 Dec;31(2 Pt 1):355-65 PMID: 6297762
  58. Isolation of transcription factors that discriminate between different promoters recognized by RNA polymerase II.
    Cell. 1983 Mar;32(3):669-80 PMID: 6187469
  59. Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit beta-globin gene in mouse 3T6 cells.
    Cell. 1983 Mar;32(3):695-706 PMID: 6299573
  60. Transcription in vivo from SV40 early promoter deletion mutants without repression by large T antigen.
    J Mol Appl Genet. 1983;2(1):127-35 PMID: 6842119
  61. Cloning and expression of a mouse adenine phosphoribosyltransferase gene.
    Gene. 1983 May-Jun;22(2-3):219-28 PMID: 6307822
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.17.8509
Published
1988-09-12
Pages
8509-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338573
Subset
IM
Grants
NCI NIH HHS · CA 36897 · United States
NIDDK NIH HHS · DK 38185 · United States
External Links
PubMed source DOI: 10.1093/nar/16.17.8509 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2